自体CIK细胞治疗肝癌的安全性和有效性评价  

Evaluation of safety and efficiency of treatment with autologous cytokine-induced killer cell for hepatocellular carcinoma

作　　者：施明[1] 王福生[1] 张冰[1] 汤紫荣[1] 雷周云[1] 王慧芬[1] 冯永毅 

机构地区：[1]解放军第302医院,北京100039

出　　处：《解放军医学杂志》2004年第4期333-335,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金 (编号 30 2 71 2 30 )资助课题

摘　　要：目的　评价原发性肝癌患者自体细胞因子诱导的杀伤 (CIK)细胞治疗的安全性及有效性。方法　采集 30例肝癌患者外周血单个核细胞(PBMC) ,在体外经干扰素 γ(IFN γ)、白细胞介素 2 (IL 2 )、抗CD3单克隆抗体 (anti CD3McAb)等诱导成CIK细胞后回输给患者 ,分析治疗前后患者外周血淋巴细胞亚群及树突状细胞(DCs)亚群的变化。结果　CIK细胞回输后 ,2 3例 (77% )患者出现发热 ,持续时间 2～8h,大部分自行消退 ,无其他不良反应。CIK细胞治疗后CD3+ 、CD3+ CD8+ 、CD3+ CD5 6 + 和CD2 5 + 细胞比例明显升高 ,而且DC1和DC2细胞的比例也上升 ,大部分患者症状明显改善。结论　肝癌患者自体CIK细胞治疗安全有效。Objective To evaluate the safety and efficiency of the treatment with autologous cytokine-induced killer (CIK) cell in patients with hepatocellular carcinoma (HCC). Methods Peripheral blood mononuclear cells (PBMC) were isolated from blood from patients with HCC, then augmented by priming with interferon-gamma (IFN-γ) followed by monoclonal antibody (mAb) against CD3 and interleukin-2 (IL-2). The autologous CIK cells thus obtained were infused back to individual patients. Altogether 30 patients undertook such treatment. The changes in subsets of lymphocytes and dendritic cells (DC1, DC2) in peripheral blood were assessed by using flow cytometry. Results The percentages of CD3 +, CD3 + CD8 + , CD3 + CD56 + , and CD25 + were increased significantly, and the proportions of DC1 and DC2 subsets were also increased after autologous CIK cells transfusion. Most (23 of 30) patients developed a fever 1-2 hours after CIK cells transfusion and the body temperature ranged from 37℃ to 40℃, lasting for 2-8 hours. Most (20 of 23) febrile patients recovered without any treatment, antipyretic medicine was given to 3 patients to allay the fever. The HCC symptoms were markedly relieved in most patients. No major side effects were found. Conclusion Our observation indicates that the treatment with autologous CIK cells for HCC patients is safe and efficacious.

关 键 词：细胞因子诱导的杀伤细胞 原发性肝细胞癌 安全性 有效性 

分 类 号：R730.3[医药卫生—肿瘤]