鼠疫耶尔森菌菌株91001的蛋白质组学初步研究  

Preliminary proteomics analysis of Yersinia pestis strain 91001

作　　者：宋亚军[1] 童宗中[2] 王津[1] 郭兆彪[1] 汪莉[3] 韩延平[1] 裴德翠[1] 周冬生[1] 王敬强[2] 李晓雷[2] 祁芝珍[4] 金丽霞[4] 戴瑞霞[4] 李敏[4] 翟俊辉[1] 杜宗敏[1] 王效义[1] 杨焕明[2] 汪建[2] 黄培堂[3] 杨瑞馥[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]中国科学院北京基因组研究所 [3]军事医学科学院生物工程研究所 [4]青海省地方病预防控制所

出　　处：《解放军医学杂志》2004年第4期315-319,共5页Medical Journal of Chinese People's Liberation Army

基　　金：国家"8 63"基金 (编号 2 0 0 1AA2 2 30 61 );国家自然科学基金 (编号30 371 2 84)资助课题

摘　　要：目的　建立鼠疫耶尔森菌的蛋白质组学研究方法 ,获得鼠疫耶尔森菌的基本蛋白质组数据。方法　以对人无致病能力的布氏田鼠疫源地菌株 910 0 1为研究对象 ,按照溶解性的不同分别收集菌体蛋白 ,以 3种不同方法 (Shotgun LC MS MS ,1D LC MS MS和2D MS)进行分析 ,将分析数据与基于 910 0 1菌株基因组全序列建立的蛋白质理论数据库进行比对 ,确定 910 0 1菌株本实验培养条件下所表达的蛋白组分。结果　Shotgun LC MS MS方法鉴定了 971种蛋白 ,1D LC MS MS鉴定了 915种 ,而 2D MS方法则鉴定了 2 33种蛋白质 ,三者合计为 1193种蛋白质 ,占基因组预测CDS的 2 8 7% (1193/414 3)。结论　不同的蛋白质组分析方法鉴定的蛋白质种类和数目都存在差异 ,为更全面地获得鼠疫耶尔森菌蛋白质组数据 ,有必要同时采取多种方法进行分析。Objective To establish reliable proteomics analysis models for Yersinia pestis and obtain the basic proteomics data of this pathogen. Methods The human-avirulent Y. pestis strain 91001 was cultured as an experimental strain, and the proteins of which were extracted and divided into three parts fractionally according to their solubility. Then three different methods (Shotgun-LC-MS-MS, 1D-LC-MS-MS and 2D-MS) were used to analyze the extracted proteins. The obtained data were compared with the theoretical protein database of strain 91001 so as to identify the expressed protein of Y.pestis strain 91001 in this study. Results 971 proteins were identified by shotgun-LC-MS-MS method, accounting for 23.4% of the predicted proteins of strain 91001 (971/4 143). 915 proteins were identified by 1D-LC-MS-MS method, accounting for 22.1% of the predicted proteins of strain 91001 (915/4 143). However, with 2D-MS only 5.62% of the predicted proteins (233/4 143) were identified. Altogether 1 193 proteins were identified when the results of the 3 methods added together, accounting for 28.7% of all the predicted CDS in 91001. Conclusion The kind and quantity of proteins identified by various proteomics methods differ from each other dramatically, therefore it is necessary to utilize multiple methods to get more reliable protein profiles of Y. pestis.

关 键 词：耶尔森氏菌 鼠疫 蛋白质组 

分 类 号：R378.61[医药卫生—病原生物学]