静脉用药后人眼房水中头孢曲松浓度的测定  

Measurement of ceftriaxone concentrations in aqueous humor of human eye after intravenous administration of the drug

作　　者：侯世科[1] 吴海洋[1] 余伟群[1] 陶海[1] 

机构地区：[1]武警总医院,北京100039

出　　处：《解放军医学杂志》2004年第4期360-361,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：目的　测定静脉用头孢曲松 1g后不同时间点人眼房水中的药物浓度。方法　将 75例白内障患者依给药后取房水时间点不同随机分为 30min、6 0min、12 0min、180min组及空白对照组 ,每组 15例。每例按所确定的时间点于术中眼内操作前取房水 2 0 0～ 30 0 μl,用高压液相色谱法测定房水中头孢曲松的浓度。结果　静脉注射头孢曲松后 30min、6 0min、12 0min、180min组的房水药物浓度分别为0 2 35± 0 12 1mg/L、0 4 74± 0 2 2 4mg/L、0 4 5 3± 0 16 2mg/L、0 5 34± 0 2 0 2mg/L。30min组的房水药物浓度低于其他组 (P <0 0 1) ,6 0min、12 0min、180min组的房水药物浓度无明显差别 (P >0 0 5 ) ,后 3组的房水药物浓度高于大部分常见致病菌的最低抑菌度 (MIC50 )。结论　头孢曲松可用于由敏感菌引起的眼内炎的治疗 ,也可作为内眼手术前的预防用药。Objective To measure ceftriaxone concentrations in aqueous humor of human eye after one gram of the drug was administered intravenously. Methods 75 subjects (75 eyes) were studied, 60 of whom were divided randomly into 4 test groups according to different time intervals between drug administration and extraction of aqueous humor (30min, 60min, 120min, 180min), and the rest 15 were controls. One-gram of ceferiaxone was administered intravenously before cataract operation. About 200-300μl aqueous humor was withdrawn from every patient during the operation at respective time point mentioned above and reversed phase high performance liquid chromatography (HPLC) was used to measure the concentration of the drug. Results The concentrations of ceftriaxone in aqueous humor were 0.235±0.121mg/L, 0.474±0.224mg/L, 0.453±0.162mg/L, and 0.534±0.202mg/L, respectively, after a time interval of 30min, 60min, 120min, 180min. The drug concentration in the 30 min group was lower compared with the other 3 test groups (P<0.01). There was no significant difference in concentration between the other 3 test groups (60min, 120min, 180min group, P>0.05), and the concentrations of ceftrixone in aqueous humor were all above the minimum inhibitory concentration for 50%(MIC 50 ) of ceftriaxone against most pathogenic bacteria. Conclusion Ceftriaxone may be an effective antibiotic for prophylactic use in ophthalmic surgery and also for the treatment of intraocular infection due to organisms susceptible to the drug.

关 键 词：头孢曲松 眼房水 色谱法 高压液相 

分 类 号：R7761.1[医药卫生—眼科] R978.1[医药卫生—临床医学]