黄芩素和大豆黄酮对人白血病细胞系HL60体外增殖的抑制作用  

Effects of Baicalein and Daidzein on proliferation of human leukemic cells HL-60 in vitro

作　　者：林小聪[1] 刘新光[1] 陈伟珠[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《右江医学》2004年第2期93-95,共3页Chinese Youjiang Medical Journal

基　　金：教育部科学技术研究重点项目 (No .0 3 0 96);广东省自然科学基金 (No .0 11766);广东省科技计划项目 ( 2 0 0 2C3 0 10 9);湛江市科技局科技攻关计划项目 ( 0 2 0 10 1)

摘　　要：目的　观察黄芩素和大豆黄酮对HL 60细胞体外增殖的抑制作用。方法　分别采用台盼蓝拒染法和MTT比色法 ,在不同条件下测定细胞增殖的抑制率。结果　MTT比色法并不适用于检测黄芩素和大豆黄酮对HL 60细胞体外增殖的影响。应用台盼蓝拒染法 ,发现黄芩素和大豆黄酮能够显著地抑制HL 60细胞的体外增殖并且呈现时效及量效关系。结论　黄芩素和大豆黄酮是两种能够明显抑制HL 60细胞体外增殖 ,具有潜在抗白血病作用的黄酮类化合物。Objective To observed the influence of baicalein and daidzein on proliferation of human leukemic cells HL-60 in vitro. Methods The inhibitory rate of cell growth was tested by MTT method and trypan blue stain assay in the various conditions.Results MTT assay was not a suitable method for this investigation. Using the trypan blue stain assay we found that baicalein and daidzein inhibited the proliferation of HL-60 cells strongly, the higher dose and/or the longer time, the more obvious effects.Conclusion Baicalein and daidzein are potential anti-leukemic flavonoid compounds which effectively inhibited the proliferation of HL-60 cell in vitro.

关 键 词：黄芩素 大豆 黄酮 人白血病细胞系 HL-60 体外增殖 抑制作用 黄酮类化合物 

分 类 号：R733.709.791[医药卫生—肿瘤]