高效液相色谱法测定赤丹退黄颗粒中芍药苷的含量  

Determination of paeoniflorin in Chidan Tuihuang Keli By HPLC

作　　者：刘峰群[1] 靳守东[2] 韩晋 

机构地区：[1]中国人民解放军第302医院,北京100039 [2]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071

出　　处：《解放军药学学报》2004年第2期134-136,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　建立赤丹退黄颗粒中芍药苷的含量测定方法。方法　高效液相色谱法 ,KromasilC1 8分析柱 ;乙腈 - 0 .1 %磷酸水溶液 (1 5∶85 )为流动相 ;检测波长 2 30nm。结果　芍药苷回归方程为Area =1 1 97.6 3×Amt.+2 1 .5 0 ,r =0 .9992 (n =6 ) ,在0 .0 5～ 2 .0 0 μg范围内呈线性关系 ,平均回收率为 1 0 2 .1 7% ,RSD为 1 .1 8%。结论　方法简便、准确、快速 ,可作为该制剂的定量方法。Aim To establish an HPLC method for the determination of paeoniflorin in Chidan Tuihuang Keli.Method The paeoniflorin was separated on a Kromasil C 18 column at 230nm by ACN-H 2O with 0.1% H 3PO 4(15∶85) as a mobile phase.Result A linear relationship of paeoniflorin was good at 0.05μg to 2.00μg.The average recovery of paeoniflorin was 102.17% with RSD 1.18%.Conclusion The method is simple,accurate and reliable for the determination of paeoniflorin in Chidan Tuihuang Keli.

关 键 词：高效液相色谱法 含量测定 赤丹退黄颗粒 芍药苷 中药 

分 类 号：R286.0[医药卫生—中药学]