乳腺癌患者外周血CEA mRNA的表达及意义  

Detection of CEA mRNA in peripheral blood from breast cancer patients by reverse transcription polymerase chain reaction

作　　者：关泉林[1] 谭继英[2] 汪晓伟[1] 高明太[2] 李玉明[2] 史大中[2] 袁文臻[1] 

机构地区：[1]兰州医学院第一附属医院肿瘤外科,兰州730000 [2]兰州医学院免疫学教研室

出　　处：《中国肿瘤临床与康复》2004年第2期109-111,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：甘肃省科学事业费资助项目(编号QS992-C33-023-14)

摘　　要：目的 检测乳腺癌患者外周血中CEA mRNA的表达,建立取材于外周血早期诊断乳腺癌微转移的方法。方法采集65例乳腺癌和37例乳腺良性疾病患者的外周血,分离其有核细胞后进行细胞总RNA的抽提,运用巢式RT-PCR技术进行CEA mRNA的检测。结果以巢式RT-PCR终产物出现131 bp带定为阳性。65例乳腺癌患者中,26例CEA mRNA阳性表达,阳性率为40.0％;37例乳腺良性疾病者CEA mRNA均无表达,差异具有显著性(x2=7.41,P<0.001)。结论RT-PCR可以用于临床检测乳腺癌患者外周血中微转移。Objective To examine carcinoembryonic antigen mRNA(CEA mRNA)in nucleated cells of peripheral blood from breast cancer patients and to establish a method for early diagnosis of breast cancer metastasis. Methods Peripheral blood samples from breast cancer patients (test group,n=65) and breast benign tumour patients (control group, n=37) were taken. Nucleated cells of the blood were separated and total RNA was extracted. CEA mRNA was detected by nested reverse transcription polymerase chain reaction (nested RT-PCR) technique.Results Samples were assessed as CEA mRNA positive when 131 bp band appeared in nested RT-PCR end-product.The positive rates of CEA mRNA was 40.0% (26/65) in test group. No benign breast tumour expressed CEA mRNA.The difference between the two groups was statistically significant (x2=7.41, P < 0.001). Conclusion RT-PCR can be used for diagnosis of breast cancer micrometastasis with relatively high sensitivity.

关 键 词：乳腺肿瘤 微转移 RT-PCR CEA MRNA 

分 类 号：R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]