托吡酯对发育期大鼠癫痫模型脑神经元损伤的保护作用  

Protective role of topiramate against neuronal damage in rat model with epilepsy during growth period

作　　者：常桂芬[1] 胡晓兰[1] 郭虎[1] 李海波[1] 

机构地区：[1]吉林大学第一医院儿科,吉林省长春市130021

出　　处：《中国临床康复》2004年第13期2468-2469,T001,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨托吡酯对发育期大鼠癫痫发作引起的脑神经元损伤的保护作用。方法:戊四氮点燃发育期大鼠癫痫模型,随机分为正常对照组、点燃模型组和托吡酯治疗组,每组27只,观察大鼠惊厥发作频率、发作程度、血清神经元特异性烯醇化酶(neuron-specificenolase,NSE)水平变化及海马区病理改变。结果:正常对照组大鼠无惊厥发作,血清NSE水平在正常范围,海马区无神经元死亡;点燃模型组大鼠惊厥出现时间早(第3天即有发作),发作程度重(第1周2级以上惊厥14只,并有3只死于惊厥),血清NSE水平犤第7,14,21天分别为(23.4±2.7),(33.4±7.8),(27.7±7.2)μg/L犦及海马区神经元死亡程度明显高于其他两组(F=1.710～5.255,P<0.05);托吡酯治疗组大鼠惊厥出现时间晚,发作程度轻(第1周2级以下轻度发作13只,2～4级中度发作4只,无重度发作),血清NSE水平犤第7,14,21天分别为(18.6±3.7),(14.9±3.7),(12.8±3.2)μg/L犦及海马区神经元死亡程度略高于正常对照组而低于点燃模型组(F=1.710～5.255,P<0.05)。结论:托吡酯对癫痫发作引起的脑神经元损伤有保护作用。AIM:To study the protective role of topiramate in neuronal damage in rat model with epilepsy during growth period. METHODS:Pentylenetetrazol(PTZ)-induced kindling in rats was used to establish rat models of epilepsy.The rats were randomly classified into 3 groups of 27 ra ts each:normal control,PTZ group and topiramate group.The degree of convulsion a nd the seizure frequency were recorded. The levels of serum neuron-specific eno lase(NSE) were measured by ELISA method and the pathological changes in hippocam pus were observed. RESULTS:In the control group, the rats had no seizure of convulsion, the level of NSE was in normal range and no neuronal death was found in the hippocampal r egion.Convulsion occurred earliest(on the 3rd day) in the rats of model group.In the first week,14 rats had convulsion more than grade 2, and 3 rats died for co nvulsion.The NSE levels in the model group were(23.4±2.7),(33.4±7.8) and(27.7 ±7.2) μg/L respectively on the 7th,14th and 21st day.The severity of convulsio n seizure,the NSE levels and the degree of neuron death in the model group were significantly higher than those in the other groups(F=1.710-5.255,P< 0.05). Con vulsion was found later in the rats of the topiramate group, and the degree of s eizure was slighter.In the first week,13 rats in the topiramate group had mild c onvulsion (less than grade 2) and 4 had moderate convulsion(grade 2-4).The leve ls of NSE were(18.6±3.7),(14.9±3.7) and(12.8±3.2) μg/L on the 7th,14th and 2 1st day.The levels of NSE and degree of neuron death in the topiramate group wer e higher as compared with those in the control group, but lower as compared with those in the model group(F=1.710-5.255,P< 0.05). CONCLUSION:Topiramate plays a protective role against epilepsy-induced neuron al damage.

关 键 词：托吡酯 发育期 大鼠 癫痫 动物模型 脑神经元损伤 保护作用 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R977.6[医药卫生—临床医学]