大鼠全脑缺血再灌注时左旋四氢巴马汀对脑内离子稳态平衡的影响(英文)  

Influence of L-tetrahydropalmatine on rat brain ion homeostasis in the course of cerebral ischemia-reperfusion

作　　者：杨光田[1] 陈齐国[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,湖北省武汉市430030

出　　处：《中国临床康复》2004年第13期2561-2563,共3页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省卫生厅基金资助项目(WJ01518)~~

摘　　要：背景:脑缺血再灌注可使脑内离子稳态遭到严重破坏,这种离子稳态的破坏又可加重脑缺血再灌注损伤。目的:通过观察脑缺血再灌注时脑组织钙、镁、锌、钠、钾、铁含量的变化,探讨左旋四氢巴马丁在全脑缺血再灌注损伤时的作用机制。设计:完全随机设计,对照实验研究。地点和材料:本研究的地点为华中科技大学同济医学院附属同济医院急诊科。材料由华中科技大学同济医学院实验动物中心提供清洁级Wistar大鼠35只,雌雄不拘,体重180～200g,鼠龄4～6周。方法:建立大鼠急性脑缺血再灌注损伤模型,用原子分光光度仪检测脑组织电解质含量。结果:与假手术组比较脑缺血再灌注12h组钙犤(218.66±11.88)μg/gq=5.526,P<0.01犦、锌犤(72.45±0.830)μg/g,q=23.240,P<0.01犦钠犤(5237.85±106.48)μg/g,q=7.956,P<0.01〗、钾犤(15421.52±264.86)μg/g,q=3.805,P<0.05犦、铁犤(151.27±10.21)μg/g,q=3.392,P<0.05犦含量增高,镁犤(617.71±13.91)μg/g,q=5.009,P0.01犦含量降低;24h钙犤(295.63±64.69)μg/g,q=12.251,P0.01犦、锌犤(74.44±0.47)μg/g,q=27.354,P<0.01犦含量进一步增高,镁犤(587.14±10.90)μg/g,q=10.499,P<0.01犦含量进一步降低钠犤(5056.68±100.18)μg/g,q=5.269,P<0.01犦、钾犤(15116.52±631.96)μg/g。BACKGROUND:Cerebral ischemia-reperfusion leads to disruption of brain ion hom eostasis that aggravates ischemia-reperfusion injury. OBJECTIVE:To investigate the action mechanism of L-tetrahydropalmatine(L-THP ) in cerebral ischemia-reperfusion injury by observing the changes in the conte nts of calcium,magnesium, zinc, sodium,potassium and iron in the brain tissues. DESIGN:Completely randomized controlled study. SETTING and MATERIALS:This study was carried out in the Emergency Department,T ongji Hospital,Huazhong University of Science and Technology.Thirty-five Wistar rats of clean grade weighing 180-200 g and aged 4-6 weeks old were used. METHODS:Rat models of cerebral ischemia-reperfusion injury were established t o determine the electrolyte contents using an atomic absorption spectrophotomete r(AAS). RESULTS:Compared with the sham operation group,the cerebral ischemia- reperfusion group, at 12 hours,had elevated levels of brain calcium[(218.66±1 1.88) μg/g,q=5.526,P< 0.01],zinc[(72.45±0.83) μg/g,q=23.240,P< 0.01}, sodium[ (5 237.85±106.48)μg/g, q=7.956,P< 0.01],potassium[(15 421.52±264.86) μg/g, q =3.805,P< 0.05]and iron[(151.27±10.21) μg/g, q=3.392,P< 0.05],but with lowered magnesium content; at 24 hours after the injury,further increases occurred in t he levels of calcium[(295.63±64.69) μg/g, q=12.251,P< 0.01],zinc[(74.44±0.47) μg/g, q=27.354,P< 0.01], sodium[(5 056.68±100.18) μg/g, q=5.269,P< 0.01]and potassium[(15 116.52±631.96) μg/g, q=4.156,P< 0.05],while the content of magne sium[(587.14±10.90) μg/g, q=10.499,P< 0.01] decreased, and the content of iron [(118.79±14.22) μg/g, q=2.515, P >0.05] was similar to that in the sham operat ion group.At both 12 and 24 hours after the reperfusion,L-THP inhibited the inc rease in the contents of calcium [(175.67±2.70) μg/g,q=3.756,P< 0.05;(190.76± 0.01;(69.94±0.98) μg/g,q=9.304,P< 0.01] and sodium[(4 841.94±179.00) μg/g, q=4.206,P< 0.05;(4 251.05±194.31) μg/g,q=3.183,P >0.05],increased the contents of magnesium [(706.21±2

关 键 词：大鼠 脑缺血 再灌注损伤 左旋四氢巴马汀 离子稳态 钙 镁 锌 钠 钾 铁 

分 类 号：R743.31[医药卫生—神经病学与精神病学]