烟草类根瘤中ATPase的活性变化及分布特性  

CHANGES AND DISTRIBUTION OF ATPase ACTIVITY IN THE PARANODULES OF NICOTIANA TABACUM

作　　者：杨雨晗[1] 韩善华[1] 陈维群[2] 

机构地区：[1]四川师范大学细胞生物学研究室,成都610068 [2]四川大学电镜室,成都610064

出　　处：《实验生物学报》2003年第4期301-306,共6页Acta Biologiae Experimentalis Sinica

基　　金：四川省教育厅重点资助项目

摘　　要：采用磷酸铅技术,对烟草类根瘤中ATPase的活性变化及分布特征进行了研究。分生细胞中没有磷酸铅颗粒,非含菌细胞的细胞质和细胞器中有少量的磷酸铅颗粒,但在年轻和成熟根瘤菌中却未见它们。相反,当非含菌细胞和根瘤菌开始衰老后,有大量的磷酸铅颗粒位于细胞的质膜和细胞壁上以及根瘤菌表面的内侧。随着它们进一步衰老,磷酸铅颗粒越来越多,广泛分布在细胞的液泡膜、质膜、细胞壁、胞间层、胞间隙及根瘤菌的表面、细胞质和拟核中。由于细胞的解体,磷酸铅颗粒明显减少,一般只位于质膜和由细胞器解体而来的膜泡状结构上。Lead phosphate deposition technique was used to investigate the changes and characteristic distribution of ATPase activity in the paranodules of tabacoo. We found that ATPase activity was closely associated with cell type and cell developmental state, thus it was different in various cells and rhizobia. No lead phosphate granules were located in the meristematic cells, although there were a small number of lead phosphate granules in the cytoplasm and organelles of the cells without rhizobia, they did not exist in the young and mature rhizobia. On the contrary, upon the senescence of the cells and rhizobia , a large number of lead phosphate granules appeared on the plasmalemmas and in the cell walls and the inside surfaces of the rhizobia. When the cells and rhizobia progressively senesced, lead phosphate granules increased in number, and they were widely distributed on the tonoplasts, plasmalemmas, cell walls, intercellular layers and in the mtercellular spaces .At the same time, they also appeared on the surfaces and in the cytoplasm and nucleoids c! the rhizobia . Due to cell disintegration, lead phosphategranules obviously decreased in number, they were only located on the plasmalemmas and membrane-vesicular structures which came from disintegrated organelles.

关 键 词：烟草 根瘤茵 细胞 ATPASE 活性 分布 生物固氮 

分 类 号：S154[农业科学—土壤学]