尼古丁是脂肪酶的反竞争抑止剂的实验研究  

Study of Nicotine Acting as an Uncompetitive Inhibitor of Lipase

作　　者：林琳[1] 陈锡侨[2] 赵福群[1] 唐应武[1] 张复实[1] 

机构地区：[1]清华大学化学系 [2]澳门大学科技学院

出　　处：《化学学报》2004年第10期1003-1006,共4页Acta Chimica Sinica

基　　金：澳门大学研究基金 (RG0 1 3/ 0 2 0 3W/CSK/FST)资助项目

摘　　要：希望明确尼古丁对脂肪酶活性是否有抑止作用 ,用于解释医学已有统计数据 :吸烟的人体重普遍较轻 ,而戒烟后体重会有回升 .用三油酸甘油酯为底物 ,在加入尼古丁前后 ,检测脂肪酶活性变化 .结果表明 :脂肪酶在人体外仍有活性 ,而且尼古丁是脂肪酶反竞争性抑止剂 .在脂肪酶浓度为 7mg/mL时 ,抑止程度达 5 0 %.The aim of the study is to determine the role of nicotine as a lipase inhibitor in fat and fatty acid metabolism and to explain the clinic phenomenon: smokers weigh less than age-matched nonsmokers, and most of smokers gain weight after smoking cessation. Lipase activity was measured in the presence or absence of nicotine using olein as substrate. Lipase is active in vitro. Nicotine is demonstrated to be an uncompetitive inhibitor of lipase at biologically reachable concentration. It is shown that the activity of lipase was reduced to 50% at lipase concentration of 7 mg/mL.

关 键 词：尼古丁 脂肪酶 反竞争抑止剂 烟草行业 生物化学反应 

分 类 号：R114[医药卫生—卫生毒理学]