DL-苯丙氨酸的酶法拆分研究  

Study on the Resolution of DL-Phenylalanine by Aminoacylase

作　　者：何仕国[1] 俞一军[1] 许文松[1] 

机构地区：[1]浙江大学化学工程与生物工程系,浙江杭州310027

出　　处：《化学反应工程与工艺》2004年第1期64-69,共6页Chemical Reaction Engineering and Technology

摘　　要：　利用氨基酰化酶能立体专一地水解氨基酰化物的特点,选择性地水解N-乙酰-L-苯丙氨酸得到L-苯丙氨酸,经分离后再水解N-乙酰-D-苯丙氨酸得到D-苯丙氨酸。研究中分别考察了pH值、温度、酶用量、底物浓度和钴离子对反应的影响。在优选条件下(底物浓度为0.1mol·L-1,pH7.0,温度37℃,酶用量为w(酶)/w(底物)=1∶41.4,加入浓度为1×10-3mol/L的Co2+,将N-乙酰-D-苯丙氨酸用有机溶剂结晶和盐酸水解10h)得到光学纯度OP=98.8%的L-苯丙氨酸,收率70.3%;OP=96%的D-苯丙氨酸,收率63%。The L-phenylalanine and D- phenylalanine has been be prepared in high optical purity by treating N-acetylphenylalanine in water using free aminoacylase as a catalyst. A number of influencing factors to the resolution reaction were discussed, including pH value, reaction temperature, amount of aminoacylase, concentration of substrate and Co^(2+). In end, L-phenylalanine was obtained with 98.8% optical purity in 70.3% yield, D- phenylalanine was obtained with 96% optical purity in 63% yield.

关 键 词：DL-苯丙氨酸 酶法拆分技术 氨基酰化酶 钴离子 浓度 纯度 收率 手性异构体 氨基酸 药物 

分 类 号：TQ464.7[化学工程—制药化工]