用单细胞凝胶电泳技术检测甲醛对小鼠睾丸细胞的DNA损伤  

Use of the Single Cell Gel Electrophoresis/Comet Assay for Detecting the DNA Damage Effect of Formaldehyde to Mouse Testicular Cell in Vivo and in Vitro

作　　者：董杰影[1] 楼哲丰[1] 郑重[2] 金龙金[1] 

机构地区：[1]温州医学院生物学实验教学中心,浙江温州325035 [2]温州医学院2000级医学检验,浙江温州325035

出　　处：《癌变．畸变．突变》2004年第3期148-150,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：浙江省温州市科技发展计划项目(No.wkw03019)

摘　　要：背景与目的 :研究甲醛对离体和体内小鼠睾丸细胞DNA的损伤作用。材料与方法 :以6、30、60、120μmol/L甲醛浓度处理离体小鼠睾丸生精细胞 ,以0.2、2、20mg/kg的甲醛腹腔暴露小鼠 ,利用单细胞凝胶电泳检测睾丸生精细胞DNA的损伤作用。结果 :甲醛在6μmol/L时就可以使小鼠离体睾丸细胞产生DNA损伤 ,与阴性对照不同(除tailmoment外) ,并随浓度增加DNA迁移也相应增加 ,到30μmol/L时DNA损伤最为明显 ,但随浓度升高DNA迁移反而减少 ,当甲醛浓度为120μmol/L时DNA迁移与阴性对照相似。腹腔注射0.2、2mg/kg的甲醛组小鼠睾丸细胞DNA迁移高于对照组 ,0.2mg/kg组DNA迁移最为明显。结论 :甲醛对小鼠睾丸细胞具有遗传性损伤 ,低剂量时是以细胞DNA断裂损伤为主 。BACKGROUND&AIM: To study the DNA damage effect of formaldehyde to mouse testicular cell in vivo and in vitro. MATERIAL AND METHODS: Mice testicular cell was exposed to6,30,60,120μmol/L dose of formaldehye in vitro,and to0.2,2,20mg/kg dose of formaldehyde five days by intraperitoneal injection.The single cell gel electrophoresis was used to detect the DNA damage. RESULTS: In vitro the DNA migration in group of6μmol/L dose obviously showed the significant damage to testicular cell DNA.30μmol/L group have the most pronounced.But the damaging effect when dose increased,DNA migration decreased by contraries.In the highest dose group(120μmol/L)there was no significance effect comparing to the concurrent negative control(P>0.05).In vivo the0.2and2mg/kg groups had the statisticsignificance against the concurrent control(P<0.01),esˉpecially the0.2mg/kg group. CONCLUSION: Formaldehyde is genotoxic to mouse testicular cell.DNA strand break is the primary DNA damage in low dose.

关 键 词：单细胞凝胶电泳 甲醛 DNA损伤 睾丸 

分 类 号：R394.33[医药卫生—医学遗传学] R994.6[医药卫生—基础医学]