大鼠一过性肠道感染模型的建立  

Development of a Transient Intestinal Infection Model in Rat

作　　者：段丽萍[1] 陈洪[1] 温燕[2] 叶嗣懋[1] 朱元莉[1] 杨燕玲[1] 

机构地区：[1]北京大学第三医院消化科,北京100083 [2]北京友谊医院热带病研究所

出　　处：《中国比较医学杂志》2003年第3期142-145,i001,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金 ( 3 0 2 40 0 3 9)

摘　　要：目的　建立大鼠一过性肠道感染模型。方法　云南大理株旋毛虫幼虫囊胞 (4 0 0 0条 1mlNaCl)感染成年雄性SD大鼠 ,分别于感染后 7、14、2 8、5 6d取大鼠小肠及结肠组织 ,4 %多聚甲醛 4℃固定过夜 ,常规石蜡浸润包埋 ,5 μm切片 ,HE染色 ,用组织形态学积分评定肠道炎症的变化。结果　大鼠于感染后 7d肠道明显充血水肿 ,糜烂 ,黏膜下出血 ;组织学观察肠黏膜绒毛破坏、融合变短 ,隐窝加深 ,绒毛隐窝比减小 ,大量中性粒细胞浸润。感染后 14d黏膜破坏程度最为严重 ,以嗜酸粒细胞浸润为主。小肠病变最为显著。感染后 2 8d肠道炎症明显减轻 ,5 6d肠道黏膜恢复正常。结论　大鼠旋毛虫感染所导致的肠道病理变化过程提供了一个理想的肠道一过性感染的动物模型 ,可用于感染后肠道功能改变的研究。Objective To develop a model of transient intestinal infection in rat.Methods\ Sprague\|Dawley male rats were infected by administering 1.0ml of normal saline containing 4000 \%T spiralis\% larvae by gavage.Rats were studied on 7,14,28,and 56 days post\|infection (PI), age matched non\|infected animals served as controls (n=4-6).Animals were killed by cervical dislocation and the tissue specimens were taken for histological assessment from jejunum,ileum,proximal colon and distal colon.Specimens were processed routinely and embedded in paraffin blocks,and 5\|μm\|think cross sections were stained with H&E and examined by light microscope.Morphometric scores were used to monitor mucosal inflammation.Results\ Microscopic examination showed hyperemia and swelling of the intestine at 7 and 14 days PI,especially in small intestine.These changes diminished at 28 days and were absent at 56 days PI.Mucosal damage was characterized by a significant decrease in villus height and increase in crypt depth,resulting in a reduced villus\|crypt ratio.Inflammatory tissue was present mainly in the mucosa and submucosa and was characterized by a prominent neutrophilic and eosinophilic infiltration at 7 and 14 days,respectively.These changes were no longer apparent by day 56 PI.Conclusion\ T spiralis infection in rat induces transient intestinal infection,so it may serves as an ideal animal model for the further study of intestinal post\|infection dysfunction.

关 键 词：大鼠 肠道感染 旋毛虫 肠道功能 肠易激综合征 IBS 

分 类 号：R574[医药卫生—消化系统] R-332[医药卫生—内科学]