Synergetic anticancer effect of combined quercetin and recombinant adenoviral vector expressing human wild-type p53,GM-CSF and B7-1 genes on hepatocellular carcinoma cells in vitro  

作　　者：MingShi Fu-ShengWang Zu-ZeWu 

机构地区：[1]DivisionofBiologicalEngineering,InstituteofInfectiousDisease,the302HospitalofPLA,Beiiing100039,China [2]InstituteofRadiationMedicine,AcademyofMilitaryMedicalSciences.Beijing100850,China

出　　处：《World Journal of Gastroenterology》2003年第1期73-78,共6页世界胃肠病学杂志（英文版）

摘　　要：AIM: This study investigated the anti-cancer effect ofcombined quercetin and a recombinant adenovirus vectorexpressing the human p53, GM-CSF and B7-1 genes(designated BB-102) on human hepatocellular carcinoma(HCC) cell lines in vitro.METHODS: The sensitivity of HCC cells to anticancer agentswas evaluated by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The viability of cells infectedwith BB-102 was determined by trypan blue exclusion. Theexpression levels of human wild-type p53, GM-CSF and B7-1genes were determined by Western blot, enzyme-linkedimmunosorbent assay (ELISA) and flow cytometric analysis,respectively. The apoptosis of BB-102-infected or quercetin-treated HCC cells was detected by terminal deoxynucleotidyltransferase (TdT) assay or DNA ladder electrophoresis.RESULTS: Quercetin was found to suppress proliferation ofhuman HCC cell lines BEL-7402, HUH-7 and HLE, with peaksuppression at 50 μmol/L quercetin. BB-102 infection wasalso found to significantly suppress proliferation of HCC celllines. The apoptosis of BB-102-infected HCC cells was greaterin HLE and HUH-7 cells than in BEL-7402 cells. Quercetin didnot affect the expression of the three exogenous genes inBB-102-infected HCC cells (P＞0.05), but it was found to furtherdecrease proliferation and promote apoptosis of BB-102-infected HCC cells.CONCLUSION: BB-102 and quercetin synergeticallysuppress HCC cell proliferation and induce HCC cell apoptosis,suggesting a possible use as a combined anti-cancer agent.AIM:This study investigated the anti-cancer effect of combined quercetin and a recombinant adenovirus vector expressing the human p53,GM-CSF and B7-1 genes (designated BB-102)on human hepatocellular carcinoma (HCC)cell lines in vitro. METHODS:The sensitivity of HCC cells to anticancer agents was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT)assay.The viability of cells infected with BB-102 was determined by trypan blue exclusion.The expression levels of human wild-type p53,GM-CSF and B7-1 genes were determined by Western blot,enzyme-linked immunosorbent assay(ELISA)and flow cytometric analysis, respectively.The apoptosis of BB-102-infected or quercetin- treated HCC cells was detected by terminal deoxynucleotidyl transferase(TdT)assay or DNA ladder electrophoresis. RESULTS:Quercetin was found to suppress proliferation of human HCC cell lines BEL-7402,HUH-7 and HLE,with peak suppression at 50μmol/L quercetin.BB-102 infection was also found to significantly suppress proliferation of HCC cell lines.The apoptosis of BB-102-infected HCC cells was greater in HLE and HUH-7 cells than in BEL-7402 cells.Quercetin did not affect the expression of the three exogenous genes in BB-102-infected HCC cells(P>0.05),but it was found to further decrease proliferation and promote apoptosis of BB-102- infected HCC cells. CONCLUSION:BB-102 and quercetin synergetically suppress HCC cell proliferation and induce HCC cell apoptosis, suggesting a possible use as a combined anti-cancer agent.

关 键 词：栎精 腺病毒载体 肝细胞癌 肿瘤细胞 肿瘤抑制 野生型P53 GM-CSF B7-1基因 

分 类 号：R730.5[医药卫生—肿瘤] R735.7[医药卫生—临床医学]