多种肿瘤细胞表面超微结构的原子力显微镜观察及药物对细胞膜表面超微结构影响的初步研究  

Observation of ultrastructures of membrane surface of various tumor cells and preliminary research on the effect of drugs on membrane surface of tumor cells by AFM

作　　者：陈勇[1] 蔡继业[1] 邢少璟[1] 张美英[1] 

机构地区：[1]暨南大学生命科学技术学院

出　　处：《电子显微学报》2003年第2期100-104,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目 (No .60 2 780 14 );国家自然科学基金重点项目 (No .30 2 30 35 0 );国家重点基础研究发展规划项目("973")基金资助 (No .2 0 0 1CB5 10 10 1);教育部科技重点项目 (No .0 0 12 4);广东省自然科学基金资助项目 (No.990 483)

摘　　要：本文应用原子力显微镜对人胃癌SGC790 1、人肝癌HepG2、人肺癌AFTC A 1、人乳腺癌MCF_7_R和人肺腺癌A5 49等肿瘤细胞膜表面进行了超微结构的形态学观察和比较。并研究了Nm2 3 H1 NDPK A蛋白对人肺腺癌A5 49等细胞膜表面超微结构的影响。发现不同肿瘤细胞膜表面超微结构存在较大的差异 ,药物处理和对照组A5 49细胞膜的表面超微结构也存在较大差异 ,药物处理的A5 49细胞膜表面出现大量的疤痕状集聚物 ,而药物处理的其它几种肿瘤细胞膜表面未发现明显的变化。说明原子力显微镜具有发展为一种观察和鉴别某些细胞的有力工具的潜力 ,并有可能应用于病理学或药理学研究 ,但在这些研究中这种技术只能作为一种辅助手段。The paper observed and compared the morphology of membrane surface ultrastructures of human gastric carcinoma SGC7901 cells, human hepatocarcinoma cell strain HepG2, human lung carcinoma AFTC-A-1 cells, human breast carcinoma multidrug-resistant MCF _7 _R cells, and human lung adenocarcinoma A549 cells by atomic force microscopy. At the same time, the effect of Nm23-1/NDPK-A protein on the membrane surface of A549 was studied. We found that there are distinct differences of membrane surface ultrastructures among tumor cells of various types and between normal A549 cells and drug-administrated A549 cells on the membrane surface of which lots of scar-like aggregations appeared. Therefore AFM has the potential as a useful assistant tool for observation or identification of some cells, and it may be applied to the study of pathology or pharmacology.

关 键 词：肿瘤细胞 超微结构 原子力显微镜 Nm23-Hl/NDPK-A蛋白 药物处理 分辨力 

分 类 号：R73-34[医药卫生—肿瘤]