脑缺血再灌注后IL-8与微血管炎症损伤关系的实验研究  

Experimental Study of IL-8 and Microvascular Inflammatory Damage after Cerebral Ischemia-reperfusion

作　　者：狄政莉[1] 万琪[2] 王洪典[2] 马铁柱[2] 

机构地区：[1]西安市中心医院神经内科,陕西西安710003 [2]第四军医大学西京医院神经内科,陕西西安710032

出　　处：《中国神经免疫学和神经病学杂志》2004年第2期87-90,F003,共5页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的　探讨白细胞介素 -8( IL-8)在大鼠全脑缺血再灌注 ( ischemia reperfusion,IR)血管内皮细胞损伤过程中的作用。方法　将大鼠随机分为假手术组和脑缺血 3 0 min再灌注 1、3、6、1 2、2 4、48、72 h组 ,动物模型采用全脑缺血模型三血管阻塞法 ,用免疫组化法检测脑组织 IR不同时间点层粘连蛋白表达以标记微血管损伤与修复过程 ,同时用原位杂交方法检测 IL-8m RNA的表达变化。结果　 IR后 1 h IL-8m RNA表达出现上调 ,2 4h达高峰。层粘连蛋白在假手术组呈强阳性表达 ,IR后 3 h表达下降 ,2 4～ 48h表达最低 ,72 h表达开始恢复。结论　 IR微血管炎症损伤主要发生在早期 ,此过程中 IL-8m RNA表达上调 ,说明 IL-8参与了脑缺血后微血管炎症损伤过程。Objective To study the role of P-selectin in microvascular inflammtory damage after cerebral ischemia -reperfusion. Methods The rats were randomly divided into the groups as sham operated control group and ischemia-reperfusion 1 h, 3 h, 6 h, 12 h,24 h, 48 h, 72 h groups. The global cerebral ischemia-reperfusion model was formed by occluding three arteries. The expression of laminin was evaluated by immunohistochemical methold, and IL-8 mRNA was detected by in situ hybridization. Results The expression of IL-8 mRNA was increased as early as 1 h after reperfusion and peaked at 24 h after reperfusion. At 72 h after reperfusion, it was still higher than that of sham operated control group. The expression of laminin was strong positive in sham operated control group.At 3 h after reperfusion, the expression of laminin began to decrease, and its lowest level was during 24-48 h. At 72 h, the expression began to reappear. Conclusions The microvascular inflammatory damage appeared in early period after cerebral ischemia-reperfusion. IL-8 was involved in the inflammatory damage of endothelial cell during global cerebral ischmia-reperfusion.

关 键 词：脑缺血再灌注 白细胞介素-8 IL-8 大鼠 IR 微血管炎症 微血管损伤 免疫组化 层粘连蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]