脂质体介导的CD44反义寡核苷酸对人眼小梁细胞粘附功能的影响  

Effect of Liposome-mediated CD44-specific Antisense Oligonucleotide on Adhesion of Human Trabecular Meshwork Cells to Hyaluronic Acid

作　　者：李中国[1] 张虹[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第2期204-206,209,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的　观察脂质体介导的CD4 4反义寡核苷酸对人眼小梁细胞粘附功能的影响 ,探讨CD4 4分子在原发性开角型青光眼 (POAG)发病过程中可能的作用。方法　采用硫代修饰的CD4 4反义寡核苷酸 ,通过脂质体导入体外培养的人眼小梁细胞 ,RT PCR、Western印迹及MTT法观察CD4 4反义寡核苷酸对人眼小梁细胞CD4 4基因表达及粘附功能的影响。结果　RT PCR及Western印迹结果提示 :CD4 4反义寡核苷酸抑制人眼小梁细胞CD4 4表达。MTT法检测显示 :CD4 4反义寡核苷酸对人眼小梁细胞与细胞外基质透明质酸的粘附起抑制作用且呈浓度依赖性。结论　CD4 4反义寡核苷酸抑制人眼小梁细胞与细胞外基质透明质酸的粘附 ,粘附分子CD4Objective To observe the effect of CD44 specific antisense oligonucleotide on adhesion of human trabecular meshwork cells to hyaluronic acid (HA) and to discuss the possible relationship between CD44 and primary open angle glaucoma (POAG). Methods CD44 specific antisense oligonucleotide was delivered with cationic lipid to cultured human trabecular meshwork cells. The expression of CD44 affected by CD44 specific antisense oligonucleotide was detected by RT PCR and Western blot. Effect of CD44 specific antisense oligonucleotide on adhesion of trabecular meshwork cells to HA was examined by MTT assay.Results The expression of CD44 was inhibited by CD44 specific antisense oligonucleotide. Antisense oligonucleotide also inhibited the adhesion of human trabecular meshwork cells to HA in a concentration-dependent manner. Conclusion The adhesion of human trabecular meshwork cells to HA was inhibited by CD44 specific antisense oligonucleotide. CD44 may play a role in pathogenesis of POAG.

关 键 词：脂质体 CD44 反义寡核苷酸 小梁细胞 细胞粘附功能 透明质酸 跨膜糖蛋白 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]