Expression of p21^(WAF1) and p53 and polymorphism of p21^(WAF1) gene in gastric carcinoma  

作　　者：Hai-LongXie Qisu Xiu-ShengHe xiao-QiuLiang Jian-GuoZhou YinSong Yi-QinLi 

机构地区：[1]InstituteofOncology,NanhuaUniversity,Hengyang421001,HunanProvince,China

出　　处：《World Journal of Gastroenterology》2004年第8期1125-1131,共7页世界胃肠病学杂志（英文版）

基　　金：Supported by the Key Programs during the 8~(th)5-Year Plan Period,the Bureau of Health,Hunan Province, China, No. 9301

摘　　要：AIM:To investigate the relationship between expression of p21^(WAF1) and p53 gene,and to evaluate the deletion and polymorphism of p21^(WAF1) gene in gastric carcinoma (GC). METHODS:Expression of p21 and p53 proteins,and deletion and polymorphism of p21 gene in GC were examined by streptavidin-peroxidase conjugated method (SP) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) respectively. RESULTS:The expression of p21 and p53 was found in 100% (20/20) and 0% (0/20) of normal gastric mucosae (NGM),92.5% (37/40) and 15.0% (6/40) of dysplasia (DP) and 39.8% (43/108) and 56.5% (61/108) of GC,respectively. The positive rate of p21 in GC was lower than that in NGM and DP (P<0.05),while the positive rate of p53 in GC was higher than that in NGM end DP (P<0.05).p21 and p53 were significantly expressed in 63.3% (19/30) and 36.7% (11/30),35.0% (14/40) and 77.5% (31/40),26.7% (4/15) and 80.0% (12/15),30.8% (4/13) and 30.8% (4/13),and 20.0% (2/10) and 30.0%,(3/10) of well-differentiated, poorly-differentiated,undifferentiated carcinomas,mucoid carcinomas and signet ring cell carcinomas.The expression of p21 in well-differentiated carcinomas was significantly higher than that in poorly-differentiated,un-differentiated, mucoid carcinomas and signet ring cell carcinomas (P<0.05). Contrarily,The expression of p53 was increased from well- differentiated to poorly-differentiated and un-differentiated carcinomas (P<0.05).The expression of p21 and p53 in paired primary and metastatic GC (35.3% and 70.6%) was different from non-metastatic GC (62.5% and 42.5%) markedly (P<0.05).The expression of p21 in invasive superficial muscle (60.0%) was higher than that in invasive deep muscle or total layer (35.2%) (P<0.05) and was higher in TNM stages Ⅰ (60.0%) and Ⅱ (56.2%) than in stages Ⅲ (27.9%) and Ⅳ (22.2%) (P<0.05),whereas the expression of p53 did not correlate to invasion depth or TNM staging (P>0.05).The exoression patterns of p53+/p21-,and of p53-/p21+ were found in 5.0% and 82.5% of DP.There To investigate the relationship between expression of p21WAF1 and p53 gene, and to evaluate the deletion and polymorphism of p21WAF1 gene in gastric carcinoma (GC).

关 键 词：P21^WAF1基因 p53基因 胃癌 基因多态性 肿瘤病理学 PCR-RFLP 

分 类 号：R735.2[医药卫生—肿瘤]