肺癌细胞高、低转移株中uPA基因启动子的序列和活性  

Sequence of uPA Gene Promoter in High and Low Metastatic Human Lung Cancer Cells and Its Biological Activity

作　　者：王丽石[1] 李平[1] 谭理[1] 朱运松[1] 

机构地区：[1]复旦大学上海医学院生物化学与分子生物学系-教育部分子医学重点实验室,上海200032

出　　处：《复旦学报（医学版）》2004年第3期243-246,共4页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金 ( 3 0 170 3 98)资助项目

摘　　要：目的　分析人肺巨细胞癌高、低转移株中uPA基因启动子序列差异和这种差异对启动子活性的影响。方法　用PCR分段扩增人肺巨细胞癌高 (95D)和低 (95C)转移细胞株中uPA基因启动子序列 ,将uPA启动子亚克隆到荧光素酶为报告基因的 pGL3 Basic载体上 ,转染 95D或 95C细胞 ,分析启动子活性。 结果　 95D与 95C细胞uPA基因启动子序列中有 3个位点碱基的差别 ,95D分别是 - 1933bp为“T” ,- 4 2 4bp为“C” ,- 96bp为“G” ,而 95C分别为“C”、“T”、“T”。这些不同对启动子活性的影响未见差别。在 - 2 14 3/ - 182 4之间存在uPA基因启动子的增强子序列 ,在 - 182 4 / - 4 0 8之间存在uPA启动子的负性调控序列。结论　uPA在 95D与 95C细胞中表达的差别不是直接由于启动子序列差别引起的 。Purpose To compare the different urokinase-type plasminogen activator(uPA) gene promoter sequences between high and low metastatic human lung cancer cells and to analyse their biological activities. Methods uPA promoter sequences from high and low metastatic human lung cancer cells were amplified from part to part using PCR method,then inserted into pGL3?Basic reporter plasmid to be detected the promoter biological activity. Results Three bases difference was discovered with 95D -1 933 bp 'T', -424 bp 'C', -96 bp 'G'.This difference did not make obviously different promoter activity when transfected and cross-transfected into two cell strains. Conclusions The different expression of uPA between the two cell strains is probably not due to the different promoter sequence bases.

关 键 词：肺癌 转移株 UPA基因 启动子 序列 活性 癌细胞 

分 类 号：R734.2[医药卫生—肿瘤]