失神经对大鼠面肌NT-3 mRNA表达影响的实验研究  

Study on Neurotrophin-3 mRNA Expression in Denervation Facial Muscles of Rats

作　　者：陈传好[1] 王震寰[1] 陈昌杰[2] 秦登友[1] 曹承亮[1] 

机构地区：[1]蚌埠医学院解剖教研室 [2]蚌埠医学院临床生化教研室,蚌埠233003

出　　处：《四川解剖学杂志》2004年第2期95-97,共3页Sichuan Journal of Anatomy

摘　　要：目的 检测神经营养素3(neurotrophin—3,NT—3)mRNA在大鼠的面肌和失神经的面肌肉的表达。方法 离断大鼠面神经的下颌缘支和颊支制备面神经损伤模型。在损伤后的第7天,采用RT—PCR技术检测NT—3mRNA在面肌和失神经的面肌内表达。结果 失神经的面肌未检测到NT—3 mRNA,有神经支配的面肌内有NT—3 mRNA的表达。结论 NT—3 mRNA在靶器官的表达可能是受其支配的神经控制。Obejective To detect the NT-3(neurotrophin-3) mRNA expression in the denervation and innervation facial muscles of rats. Methods The model of facial nerve le- sion was established in rats by cutting the marginal madibular branch and buccal branches of facial nerve. RT-PCR was employed to confirm whether NT-3 mRNA expressed in the denervation and innervation facial muscles of rats 7 days after lesion. Results Compared with NT-3 mRNA expression in the innervation facial muscles of rats, the NT-3. mRNA expression were not detected in the denervation facial muscles of rats. Conclusion the NT-3 mRNA expression in target organs was possibly controlled by neurons factor.

关 键 词：失神经 大鼠 面肌 NT-3 MRNA表达 实验 神经营养素3 周围神经损伤 

分 类 号：R363[医药卫生—病理学]