The Fate of Mitochondria in Ibex-hirus Reconstructed Early Embryos  

作　　者：YanJIANG Shu-ZhenLIU Yan-LingZHANG Man-XiJIANG Qing-YuanSUN Da-YuanCHEN 

机构地区：[1]StateKeyLaboratoryofReproductiveBiology,InstituteofZoology,theChineseAcademyofSciences,Beijing100080,China

出　　处：《Acta Biochimica et Biophysica Sinica》2004年第5期371-374,共4页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the Special Funds for Major Basic Research Project of China (G 20000161)

摘　　要：Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endan- gered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endan- gered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.

关 键 词：Capra ibex cloned embryos nuclear transfer MITOCHONDRIA PCR 

分 类 号：Q959.842[生物学—动物学] Q78