软骨细胞三种凋亡模型的建立  

Establishment of Three Models on Chondrocyte Apoptosis

作　　者：张梅[1] 李平[1] 汪健[1] 翟志敏[1] 胡闻[1] 陈朝晖[2] 

机构地区：[1]安徽省立医院 [2]安徽中医学院,合肥230001

出　　处：《上海实验动物科学》2004年第1期11-15,60,共6页Shanghai Laboratory Animal Science

基　　金：国家中医药管理局课题(2000-JP-58)

摘　　要：为了观察药物对骨关节炎中软骨细胞凋亡的影响,建立了软骨细胞三种凋亡模型。将硝普钠(SNP)、全反式维甲酸(ATRA)、肿瘤坏死因子-α(TNF-α)分别加入软骨细胞培养体系,建立三种不同药物诱导的软骨细胞凋亡模型,采用Annexinv/PI双参数法以及透射电镜对软骨细胞凋亡进行定量、定性检测。在兔软骨细胞培养体系中分别加入2mmol/LSNP,10-4mol/LATRA24h后,在人胚胎软骨细胞培养体系中加入30μg/L人TNF-α24h后,均可成功建立软骨细胞凋亡模型。提示硝普钠、全反式维甲酸、肿瘤坏死因子-α加入软骨细胞培养体系中,可建立软骨细胞凋亡模型。To investingate the effect of drug on apoptosis of chondrocyte in osteoarthritis, the three chondrocyte apoptosis models were established,treated with sodium nitroprusside(SNP) 、all-trans retinoic acid (ATRA)and tumor necrosis factor-α (TNF-α) respectively. The apoptosis rate were analysed by AnnexinⅤ/PI with flow cytometry and the characteristic alterations of morphology were detected by electron miscroscope.When rabbit chondrocyte were incubated with SNP(2mmol/l),and ATRA(10^(-4) mmol/l), and chondrocyte coming from fetal were incubated with TNF-α(30 μg/L) for 24h,they all could induce apoptosis in chondrocyte in vitro. The results suggested that the chondrocyte apoptosis models could be established by sodium nitroprusside、all-trans retinoic acid and tumor necrosis factor-α respectively.

关 键 词：软骨细胞 凋亡 模型 骨关节炎 药物 

分 类 号：R-332[医药卫生]