封闭群KM小鼠生物净化前后免疫功能指标观察  

Changes of Immune Function in Closed Colony of KM Mice after Biocleaning

作　　者：卢笑丛[1] 林乔[2] 王玉娥[2] 王有为[1] 

机构地区：[1]中国科学院武汉植物研究所,武汉430074 [2]湖北省预防医学科学院,武汉430079

出　　处：《上海实验动物科学》2004年第1期23-25,29,共4页Shanghai Laboratory Animal Science

基　　金：湖北省科技厅"九五"重点科技发展项目(972P0904)

摘　　要：为了研究从普通级净化到 SPF级前后的 KM小鼠免疫功能变化的特点 ,对同一封闭群的KM小鼠在净化前后分别进行免疫功能的测定。结果 KM小鼠净化前后抗体生成细胞分别是5 5 7.39± 85 .77和 368.62± 65 .2 3;血清抗体水平分别是 67.8± 2 6.0 8和 46.4± 1 5 .45、碳廓清指数 K分别是 5 .2 4± 0 .96和 4.61± 0 .61 ;碳廓清吞噬指数 α分别是 0 .0 32± 0 .0 2 3和 0 .0 2 5± 0 .0 1 ;小鼠巨噬细胞吞噬鸡红细胞的指数分别为 0 .41± 0 .0 6和 0 .36± 0 .0 6,吞噬百分数分别为 38.42± 6.1 5和31 .5 5± 5 .75 ;Con A诱导小鼠脾淋巴细胞转化能力 OD值分别为 0 .0 32± 0 .0 2和 0 .0 31± 0 .0 1 ;二硝基氟苯诱导小鼠迟发型变态反应 (耳肿胀法 )小鼠耳重差分别为 1 0 .85± 4.34mg和 1 6.2 9± 4.2 1mg。结果表明 ,净化后的 KM小鼠的巨噬细胞活力、B淋巴细胞活性、抗体水平均小于净化前 ,有极显著的差异 ( P<0 .0 1 ) ;二硝基氟苯诱导小鼠迟发型变态反应 ( DHT)大于净化前 ,有极显著的差异 ( P<0 .0 1 ) ;Con A诱导小鼠脾淋巴细胞转化能力没有变化。In order to observe the changes of immune function in KM mice before and after biocleaning, immunological examinations were conducted. The function of antibody formation cells was 557.39±85.7 and 368.62±65.23, the antibody level in serum was 67.8±26.08 and 46.4±15.45, the function of removing carbonaceous granule index K was 5.24±0.96 and 4.61±0.61, α was 0.0032±0.0023 and 0.0025±0.001, the phagocytic function of macrophages in mice's abdominal cavities 0.41±0.66 and 0.36±0.06, the mice's lymphocyte transformation induced by ConA (OD value) 0.023±0.02 and 0.031±0.001,the mice's DTH induced by DNFB 10.85±4.34 and 16.29±4.21 before and after biocleaning respectively. It was suggested that some of examined immune function changed significantly after biocleaning such as the phagocytic activity, B lymphatic cells activity and antibody levels. However, no changes were observed in mice's lymphocyte transformation induced by ConA.

关 键 词：封闭群 KM小鼠 生物净化 免疫功能 实验动物 生物医学研究 

分 类 号：R-332[医药卫生]