青霉DNA提取方法比较研究  

Comparision of Methods of Extracting DNA from Penicillium

作　　者：崔丽霞[1] 韩建荣[1] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2004年第2期185-187,共3页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金 (30 0 70 0 2 1)

摘　　要：比较了 CTAB、SDS- CTAB和氯化苄等 3种 DNA提取方法提取青霉 DNA的效果 ,结果表明 :CTAB法和SDS- CTAB法只适合于部分青霉菌株的 DNA提取 ,另外一些菌株则提取不出谱带清晰的 DNA.而氯化苄法适合于供试的所有青霉菌株的 DNA提取 ,所有菌株的 DNA凝胶电泳图谱带清晰 ,效果很好 .此外 ,还研究了液氮研磨和提取液 p H对氯化苄法提取Three kinds of methods(i.e.benzyl choeide method, CTAB method and SDS-CTAB method) were used to extract DNA from ten strains of Penicillium.The results showed that the benzyl choeide method was able to be used to extract the DNA of those Penicillium strains, and bands resulting from gel electrophoretic separations were very clear. However, both the CTAB and SDS-CTAB methods were only adequate to be used to extract DNA of some strains of Pencicillium, and the DNA bands of some strains were clear, but others were not obvious. The effects of both the pH values of extracts and the grinding of mycelia in liquid nitrogen on the result of DNA extracting were also discussed.

关 键 词：青霉 DNA 提取方法 氯化苄 丝状真菌 

分 类 号：Q939.11[生物学—微生物学] Q781