Q Sepharose F.F.层析柱的制备及BTI的初步纯化  

Q Sepharose F.F. Anion Exchange Chromatography Column Packing and Preliminary Purification of Buckwheat Trypsin Inhibitor

作　　者：赵卓慧[1] 李玉英[1] 张政[1] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2004年第2期192-194,共3页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省科技厅攻关项目 (0 110 0 1)

摘　　要：通过对 Q Sepharose F.F.离子柱的制备及荞麦蛋白酶抑制剂 (BTI)的初步纯化 ,探索用自制层析柱代替价格昂贵的预装柱来分离纯化生物活性物质的可能性 .实验结果表明 :自制的 Q Sepharose F.F.强阴离子层析柱可以满足对目的产品进行分离纯化的实验要求 ,同时与购买的预装柱比较 ,自装柱具有较大的上样量 。Ion-exchange chromatography is one of the most important methods for separating and purifying biomolecules including natural proteins and recombinant proteins.In order to explore the possibility of separating biomolecules with self-packed chromatography column,we packed a Q Sepharose Fast Flow anion exchange chromatography column and preliminarily purified buckwheat trypsin inhibitor.When comparing the chromatography efficiency with pre-packed Resource Q column,it showed that the packed Q Sepharose Fast Flow anion exchange chromatography column could meet the requirements for separating target protein,and it was superior to the pre-packed column in greater sample volume,more flexible column volume and lower cost.

关 键 词：Q SEPHAROSE F.F.层析柱 制备 BTI 纯化 胰蛋白酶抑制剂 离子交换层析 

分 类 号：Q814.1[生物学—生物工程]