SO_2体内衍生物对小鼠胃细胞DNA损伤的研究  

Damage Effects of Sulfur Dioxide Derivatives Injection on DNA of Stomach Cells from Mice

作　　者：李瑞金[1] 孟紫强[1] 张波[2] 

机构地区：[1]山西大学环境医学与毒理学研究所,山西太原030006 [2]北京联合大学应用文理学院生物系,北京100038

出　　处：《山西大学学报（自然科学版）》2004年第2期205-208,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金 (No.30 0 70 6 4 7) ;山西省自然科学基金 (No.99112 3)

摘　　要：SO2 体内衍生物是 SO2 进入机体后在体液中代谢转化的衍生物—亚硫酸盐和亚硫酸氢盐 .运用单细胞凝胶电泳技术 (又称慧星试验 )研究了 SO2 体内衍生物对小鼠腹腔注射引起小鼠胃细胞 DNA的影响 .在衍生物剂量为 0 g/ kg体重 ,0 .12 5 g/ kg体重 ,0 .2 5 0 g/ kg体重 ,0 .5 0 0 g/ kg体重的染毒条件下 ,雄性小鼠胃细胞 DNA拖尾长度分别为 1.14 μm,8.98μm,14 .33μm,2 4 .71μm,雌性小鼠胃细胞 DNA拖尾长度分别为 1.2 0 μm,6 .34μm,12 .6 7μm,18.5 9μm.各处理组与对照组的拖尾长度有显著性差异 .结果表明 ,SO2 衍生物可引起小鼠胃细胞 DNA损伤 ,且随 SO2 体内衍生物注射剂量的增高 DNA损伤加重 ,并有明确的剂量效应关系 (r>0 .95 ) .另外 SO2 体内衍生物对雌性小鼠胃细胞 DNA损伤比雄性小鼠弱 .这些意味着 SO2 的体内衍生物具有引起哺乳动物胃细胞Sulfite and hydrosulfite are two kinds of sulfur dioxide derivatives in vivo.The damage effects of sulfur dioxide(SO_2)derivatives injection on DNA of stomach cells from mice were studied with the single cell microgel electrophoresis technique(Comet test).After exposure to SO_2 derivatives at 0 g/kg body weight,0.125 g/kg body weight,0.250 g/kg body weight,g/kg body weight,tail lengths of nuclear DNA of stomach cells from male mice were 1.14 μm,8.98 μm,14.33 μm,24.71 μm,and female mice were 1.20 μm,6.34 μm,12.67 μm,18.59 μm,respectively.The results showed that DNA damage of stomach cells was in a dose-dependent manner.The results indicated that DNA of stomach cells was sensitive to SO_2 derivatives,and DNA damage of the stomach cells from male mice was more serious than that from female mice,which remains to be further studied.These results lead us to conclusion:SO_2 pollution has a potential risk to damage genetic material DNA of stomach cells from mammalian animals.

关 键 词：二氧化硫体内衍生物 DNA损伤 胃 小鼠 慧星试验 二氧化硫 大气污染物 

分 类 号：X511[环境科学与工程—环境工程] R-33[医药卫生]