内氨酰组氨酸对脑内组胺含量以及癫痫行为的影响  

Effect of alahistidine on brain histamine content and seizure development

作　　者：胡薇薇[1] 陈忠[1] 徐莉莎[1] 金春雷[1] 樱井映子[2] 谷内一彦[2] 

机构地区：[1]浙江大学医学院药理学教研室,浙江杭州310031 [2]日本东北大学医学部药理教研室

出　　处：《浙江大学学报（医学版）》2004年第3期197-200,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金 ( 30 3716 38);浙江省青年人才基金( R30 3779)资助项目

摘　　要：目的 :研究内氨酰组氨酸对脑内组胺以及大鼠癫痫行为的作用。方法 :大鼠隔日腹腔注射亚惊厥剂量的戊四唑 ( PTZ,35 mg/kg)诱发化学点燃癫痫。利用 HPL C法和化学荧光法测定脑内组胺和单胺类物质的含量。结果 :给予大鼠低组胺食物 2周后 ,其大脑皮层和下丘脑内组胺含量明显减少 ,同时其 PTZ癫痫的形成显著加快。内氨酰组氨酸可以逆转服用低组胺食物 2周诱发的组胺下降以及减慢 PTZ癫痫的诱发。无论是低组胺组还是喂于内氨酰组氨酸食物组都不影响大鼠大脑皮层和下丘脑内的去甲肾上腺素、多巴胺含量以及多巴胺的代谢。结论 :食物中的内氨酰组氨酸可影响大鼠脑内组胺神经系统并进一步调节癫痫等行为。Objective: To investigate the effect of alahistidine on brain histamine content and seizure development. Methods: The kindling seizure was induced by ip injection with subconvulsant dose of pentylenetetrazole every 48 h. Monoamines and their metabolites were measured using a HPLC system and fluorometric assay. Results : Chronic low histamine feeding markedly decreased histamine content in cortex and hypothalamus, and promoted seizure development induced by pentylenetetrazole. However, alahistidine feed reversed the decreased histamine content and slowed seizure development caused by low histamine feed. Both low histamine and alahistidine feed had no effect on norepinephrine, dopamine and its metabolites. Conclusion: Alahistidine may affect histaminergic system and seizure development.

关 键 词：内氨酰组氨酸/药理学 组胺/分析 癫痫/化学诱导 戊四唑 

分 类 号：R742.1[医药卫生—神经病学与精神病学]