脑内组胺对戊四唑慢性癫痫形成过程的作用机制  

Mechanisms of the effect of brain histamine on chronic epilepsy induced by pentylenetetrazole

作　　者：张力三[1] 沈海清[1] 金春雷[1] 胡薇薇[1] 赵孟辉[1] 陈忠[1] 

机构地区：[1]浙江大学医学院药理学教研室,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2004年第3期201-204,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金( 30 0 0 0 0 19;30 3716 38);浙江省青年人才基金 ( R30 3779)资助项目

摘　　要：目的 :探讨组胺对戊四唑诱发慢性癫痫形成的作用机制。方法 :大鼠隔日腹腔注射亚惊厥剂量的戊四唑( 35 mg/ kg)诱发化学点燃癫痫。观察每次戊四唑注射后 30 min内的癫痫发作行为变化。结果 :腹腔内注射组氨酸或侧脑室内注射 clobenpropit都能剂量依赖性地延缓癫痫的形成 ,表现为延长肌性痉挛、阵挛性癫痫全身性发作的反应潜伏时间及抑制癫痫发作的级别。 H3 受体激动剂 immepip和组氨酸脱羧酶抑制剂α-氟甲基组氨酸剂量依赖性地抑制 clobenpropit的抗癫痫作用。结论 :脑内组胺在对抗阵挛性癫痫发作中起较重要的作用 ,其作用主要与Objective: To investigate the mechanisms of histamine on chronic epilepsy induced by pentylenetetrazole (PTZ). Methods: To induce chemical kindling, a subconvulsive dose (35 mg/kg) of PTZ was ip injected every 48 h in rats. Behavior changes were observed for 30 min after every injection of PTZ. Results: Ip injection of histidine or icv injection of clobenpropit inhibited the development of kindling induced by PTZ, presenting prolonged latency for myoclonic jerks and clonic generalized seizures and depressed seizure stages in a dose-dependent manner. H 3-receptor agonist, immepip, and histidine decarboxylase, α-fluoromethylhistidine reversed the ameliorating effect of clobenpropit on seizure development in a dose-dependent manner. Conclusion: Brain histamine plays an important role in protection against myoclonic jerks and clonic generalized clonic seizures and its action may be via H3-receptor.

关 键 词：癫痫/化学诱导 组氨酸/药理学 H3受体 组胺 戊四唑 

分 类 号：R742.1[医药卫生—神经病学与精神病学]