灵芝三萜类化合物高效液相指纹图谱研究  

HPLC Fingerprinting of Triterpenoids from Ganoderma Lucidum

作　　者：黄书铭[1] 杨新林[1] 黄健[1] 吴建华[1] 徐建兰 朱鹤荪[3] 

机构地区：[1]北京理工大学生命科学与技术学院 [2]无锡市三联高科技开发有限公司,江苏无锡214023 [3]北京理工大学材料科学与工程学院,北京100081

出　　处：《北京理工大学学报》2004年第5期458-461,共4页Transactions of Beijing Institute of Technology

基　　金：国家重点科技攻关项目(1999)

摘　　要：建立灵芝中所含三萜类组分(triterpenoidcomponents,TC)的高效液相指纹图谱.利用Agilent1100高效液相色谱仪对10批次灵芝TC样品进行了分析.色谱条件为:ZORBAX300SBC18分析柱(5μm,250.0mm×4.6mm),流动相为甲醇和质量分数为5%的醋酸(体积比4∶6)混合溶液,检测波长252nm.结果显示,10批次TC样品均出现8个主要特征峰,相对保留时间α分别为0.69,0.78,0.88,0.92,1.00,1.27,1.48和1.61,并且10批次TC样品的8个主峰面积均在70%以上,重叠率均在90%以上.A sensitive and specific HPLC fingerpringting (FP-HPLC) of triterpenoid components (TC) from Ganoderma lucidum was establised. Agilent 1100 series chromatographic setup was used as HPLC analyzer to detect 10 lots of TC samples. Chromatographic conditions in use were: ZORBAX 300SB C(18) column (5 μm, 250.0 mm×4.6 mm), methanol in 5% acetic acid solution (V∶V=40∶60) as the mobile phase, and 252 nm as the detection wavelength. Eight main characteristic peaks were selected from the HPLC fingerprinting of 10 lots of samples, with relative retention time α values of 0.69,0.78,0.88,0.92,1.00,1.27,1.48,1.61 respectively. Eight among the peak areas for the 10 lots of samples were higher than 70% and all the overlapping ratios were higher than 90%.

关 键 词：灵芝 三萜化合物 高效液相色谱 指纹图谱 

分 类 号：TQ460.72[医药卫生—药物分析学]