模拟胃癌外周血微转移免疫磁珠检测法的建立  

To establish IMB examination methods of the model of micrometastases in peripheral blood of gastric cancer

作　　者：王文秀[1] 方立萍[1] 路丹[1] 信涛[1] 徐玉清[1] 

机构地区：[1]哈尔滨医科大学附属第二医院肿瘤内科,黑龙江哈尔滨150086

出　　处：《中国癌症杂志》2004年第2期113-115,共3页China Oncology

基　　金：黑龙江省九五攻关课题(G98c19-21)。

摘　　要：目的:探讨建立胃癌外周血微转移癌细胞的免疫磁珠检测法及其意义。方法:体外培养胃癌SGC-7901细胞,将胃癌细胞与PBMC及全血细胞混合,细胞混合液与单克隆抗体(CA199、CEA单抗)在4℃下作用90分钟,然后与免疫磁珠(M-450)作用60分钟后,在磁性细胞分离器中分离,涂片,HE染色,计算玫瑰花环形成率,并进行灵敏度的检测。结果:免疫磁珠技术敏感性高,当PBMC与SGC-7901细胞比为5×105:1时可检测到恶性细胞。1 ml外周血中有20个肿瘤细胞即可被检测出来。结论:免疫磁珠技术是一种简便、快速、敏感性强且临床实用价值高的检测技术,可提高胃癌转移的早期诊断率。Purpose: To study the method of IMB examination of circulating gastric cancer cell in the peripheral blood and its significance. Methods: Gastric cancer cell line( SGC-7901) cultured in vitro, mixed with PBMC and all blood cells. The malignant cell mixtures were incubated with Mab ( CA199, CEA Mab), 90 minutes and then reacted with immunomagnetic beads ( Dynabeads M-450) for 60 minutes. After isolated through magnetic fields , the cells were observed through microscope and smeared and then HE-dyed . The rosette formatting rate was counted , then to evaluate the sensitive of IMB. Results: We can detect one cell in 5×105:1 PBMC and we can also detect the malignant cells when there are more than 20 SGC-7901 cells in one milliliter liquid. Conclusions: IMB-technique is a high sensitive, rapid, simple detective technique and can improve the early diagnosis rate of metastases in gastric cancer.

关 键 词：胃癌 微转移 免疫磁珠 单克隆抗体 

分 类 号：R735.2[医药卫生—肿瘤]