Toosendanin increases free-Ca^(2+) concentration in NG108-15 cells υia L-type Ca^(2+) channels  

作　　者：Tong-huiXU junDING Yu-liangSHI 

机构地区：[1]KeyLaboratoryofNeurobiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China [2]CorrespondencetoProfYu-liangSHI

出　　处：《Acta Pharmacologica Sinica》2004年第5期597-601,共5页中国药理学报（英文版）

基　　金：Project supported by the National Basic Research Program ofChina (No G1999054000); National Natural Science Founda-tion of China (No 39870249 and 30170302); and the Basic Re-search Program (No 02JC14011).

摘　　要：AIM: To examine if toosendanin (TSN) affects intracellular free-Ca2+ concentration ([Ca2+]i) in neuroblastomagliomahybrid cells (NG108-15 cells). METHODS: The [Ca2+]i was determined by laser-scanning confocal microscopicimaging technique in which Fluo-3 was used as Ca2+ indicator. RESULTS: TSN induced an increase in resting[Ca2+]i and in high K+-evoked Ca2+ transient in differentiated NG108-15 cells. The TSN-induced increase in [Ca2+]iwas dose-dependent and disappeared in CdCl2-, nifedipine-containing or Ca2+-free solution, and appeared afterwashing out the Ca2+ channel blockers or adding Ca2+. CONCLUSION: TSN increased [Ca2+]i in differentiatedNG108-15 cells. The [Ca2+]i enhancement was due to the influx of extracellular Ca2+ and related to L-type Ca2+channels.AIM: To examine if toosendanin (TSN) affects intracellular free-Ca2+ concentration ([Ca2+]i) in neuroblastomagliomahybrid cells (NG108-15 cells). METHODS: The [Ca2+]i was determined by laser-scanning confocal microscopicimaging technique in which Fluo-3 was used as Ca2+ indicator. RESULTS: TSN induced an increase in resting[Ca2+]i and in high K+-evoked Ca2+ transient in differentiated NG108-15 cells. The TSN-induced increase in [Ca2+]iwas dose-dependent and disappeared in CdCl2-, nifedipine-containing or Ca2+-free solution, and appeared afterwashing out the Ca2+ channel blockers or adding Ca2+. CONCLUSION: TSN increased [Ca2+]i in differentiatedNG108-15 cells. The [Ca2+]i enhancement was due to the influx of extracellular Ca2+ and related to L-type Ca2+channels.

关 键 词：TOOSENDANIN intracellular calcium concentration confocal microscopy calcium indicator neuroblastomaglioma hybrid cells L-type Ca2+ channels 

分 类 号：R972[医药卫生—药品]