耐药基因蛋白在结直肠癌组织中的表达及其临床意义  

Expression and Clinical Significance of Drug Resistance Gene Associated Proteins in Colorectal Carcinoma

作　　者：张建兵[1] 何松[1] 缪宏兰[1] 吴丽华[1] 韩枋[1] 于兰[1] 杨书云[1] 周建云[1] 

机构地区：[1]江苏省南通市肿瘤医院病理科,226361

出　　处：《实用癌症杂志》2004年第2期162-165,174,共5页The Practical Journal of Cancer

摘　　要：目的　探讨结直肠癌组织中P 糖蛋白 (P gp)、谷胱甘肽 S 转移酶 (GST π)、拓扑异构酶Ⅱ (TopoⅡ )和胸苷酸合成酶(TS)的表达及临床意义。方法　应用免疫组化方法检测 65例随访 10年以上患者的结直肠癌组织中P gp、GST π、TopoⅡ和TS的表达 ,并分析其与临床预后的关系。结果　GST π的表达与UICC分期有关 (P =0 .0 2 18) ,与肿瘤组织类型、浸润深度及淋巴结转移无关 ;P gp、TopoⅡ和TS的表达与上述参数均无关 (P >0 .0 5 )。经多因素Cox比例风险模型分析显示 ,UICC分期、肿瘤组织类型及GST π、TopoⅡ的表达与结直肠癌患者术后生存率有关 (P <0 .0 5 )。 结论　采用免疫组化方法检测结直肠癌组织中P gp、GST π、TopoⅡ和TS的表达 ,对肿瘤化疗药物的选择具有重要临床意义 ;UICC分期、组织类型及GST π。Objective To investigate the expression and clinical significance of P-glycoprotein(P-gp),glutathione-S-transferase-π(GST-π),topoisomerase Ⅱ(TopoⅡ),and thymidylate synthase(TS)in colorectal carcinoma.Methods The expressions of P-gp、GST-π、TopoⅡ,and TS proteins were detected by immunohistochemistry method in sixty-five patients with colorectal carcinoma who were followed up more than 10 years.The relationship between the expression of drug resistance proteins and clinical prognosis were analysed.Results The expression of GST-π was associated with UICC staging(P=0.0218),and wasn′t associated with histological grading,infiltration depth and lymph node status.The expression of P-gp、TopoⅡ and TS were not related to the above mentioned parameters(P>0.05).In the multivariate Cox analysis,survival rate was correlated with UICC staging,histological grading,GST-π,and TopoⅡ(P<0.05).Conclusion The detection of the expression of P-gp、GST-π、TopoⅡand TS by immunohistochemistry has important clinical significance for the selection of chemotherapy regimen.UICC staging,histological grading,and the expression of GST-π and TopoⅡ can be as independent prognostic factors in colorectal carcinoma.

关 键 词：耐药基因蛋白 结直肠癌 癌组织 临床意义 肿瘤浸润 肿瘤转移 

分 类 号：R735.3[医药卫生—肿瘤]