α粒子辐射诱导人肺鳞癌裸鼠转移模型的建立  

Establishment of a metastatic model of human lung carcinoma induced by α particles in nude mice

作　　者：胡迎春[1] 项晓琼[1] 徐勤枝[1] 霍艳英[1] 李邦印[1] 段瑞峰[1] 李刚[1] 张开泰[1] 吴德昌[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2004年第1期16-18,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家重点基础研究专项资金资助项目 (G19980 5 12 0 7)

摘　　要：目的　建立α粒子辐射诱导人肺鳞癌裸鼠转移模型。方法　α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种裸鼠皮下 ,后采用组织块接种的方法 ,在鼠间连续传代 3次 ,计算原代成瘤率及传代成活率 ,并观察肿瘤的转移情况 ,病理切片和免疫组织化学鉴定肿瘤的性质和来源。结果 (1)α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种成瘤率为 4 4 4 % (4 9) ;(2 )原代肿瘤的体表转移率与肺转移率均为 2 5 % (1 4 ) ;(3)病理切片鉴定为低分化鳞状上皮细胞癌 ;(4)免疫组织化学显示CK3和EMA表达阳性。结论　采用组织块接种的方法 ,在α粒子辐射诱导人肺鳞癌细胞系BERP35T4的基础上 ,经过裸鼠体内连续传代 3次 ,成功地建立了人类肺癌裸鼠转移模型。Objective To establish a metastatic model of human lung carcinoma induced by α particles in nude mice. Methods A total of 9 BALB/C nude mice (5-6 weeks old) was inoculated subcutaneously (SC) with human tumor cell line BERP35T4.The tumor nodules developed from the nude mice were passaged for 3 times in nude mice via orthotopic implantation of histologically intact tissues. Results (1)Four of 9 nude mice developed (44 ^4%)neoplasm at the initial inoculation site.(2)one of four nude mice with neoplasm (25%) presented subcutaneous and lung metastasis.(3)Tumor tissue isolated from nude mice was characterized as squamous epithelial carcinoma with low differentiation.(4)CK3 and EMA staining were positive in cancer cells. Conclusion {A metastatic } model of human lung carcinoma induced by α particles in nude mice has been established via orthotopic implantation of histologically intact tissues. ;

关 键 词：Α粒子 辐射损伤 诱导 肺鳞癌 裸鼠转移模型 免疫组织化学 

分 类 号：R-332[医药卫生]