组织因子表达对原发性结直肠癌侵袭及转移能力的影响  

The role of tissue factor expression in the invasive and metastatic ability of colorectal carcinoma

作　　者：万远廉[1] 姚宏伟[1] 叶京明[1] 刘玉村[1] 吴涛[1] 汪欣[1] 潘义生[1] 吴楠[1] 鞠晓明[1] 朱静[1] 黄莚庭[1] 

机构地区：[1]北京大学第一医院普通外科,100034

出　　处：《中华外科杂志》2004年第3期149-153,共5页Chinese Journal of Surgery

基　　金：2 0 0 2年教育部科学技术研究重点项目基金资助项目 ( 0 2 0 0 3 )

摘　　要：目的　探讨组织因子 (TF)表达在原发性结直肠癌侵袭及转移中的作用 ,分析TF对人类大肠癌HT 2 9细胞侵袭能力的影响。方法　应用免疫组织化学染色法研究 85例原发性结直肠癌和 6例结直肠良性腺瘤标本TF表达情况 ,分析TF表达与肿瘤侵袭转移及预后的关系 ;利用构建有正义 /反义TFcDNA的质粒pcDNA3 1/Zeo ,以脂质体法转染HT 2 9细胞 ;转染成功的HT 2 9细胞采用Western印迹分析检测TF表达水平并进行基质胶体外侵袭实验观察细胞侵袭能力的变化。结果85例结直肠癌标本中 4 0例 (4 7 1% )TF表达阳性 ,正常黏膜和结直肠良性腺瘤均为TF阴性表达 ;TF阳性表达与结直肠癌的浸润深度密切相关 (r =0 895 ,P <0 0 1) ;TF阳性表达与结直肠癌的同时性 (r =0 974 ,P <0 0 1)和异时性 (r =0 96 3,P <0 0 1)肝转移均密切相关 ;Logistic回归表明TF表达为结直肠癌肝转移的影响因素 (P <0 0 1) ,多因素回归分析表明TF表达是影响原发性结直肠癌预后的因素之一 (P <0 0 1) ;转染了正义TFcDNA的HT 2 9细胞的TF表达水平较未转染的HT 2 9细胞升高 ,转染了反义TFcDNA的HT 2 9细胞的TF表达水平则降低 ;转染了正义TFcDNA的HT 2 9细胞的侵袭能力较未转染的HT 2 9细胞增强 ,转染了反义TFcDNA的HT 2 9细胞的侵袭能力则减弱。结论TF?Objective To investigate the role of tissue factor(TF) expression in the invasive and metastatic ability of colorectal carcinoma and explore the influence of TF on the invasive ability of HT 29 cells. Methods TF expression of specimens from 85 colorectal carcinomas and 6 colorectal adenomas was observed by immunohistochemistry. The role of TF expression in prognosis and tumor invasion and metastasis was analyzed. The plasmids pcDNA3 1/Zeo bearing either sense or antisense TFcDNA were transfected into HT 29 cells by the way of Lipofectamine 2000. TF proteins in transfected and untransfected HT 29cells were detected by Western blot. In vitro Matrigel invasion assays were performed to show the invasive ability of those cells. Results TF expression was positive in 40 (47 1%) of 85 colorectal carcinoma specimens, but negative in normal mucosa and adenoma specimens. TF expression showed significant correlation with tumor invasive depth ( r =0.895, P <0 01). TF expression showed significant correlation with synchronous and metachronous hepatic metastasis ( r =0.974, P <0 01 and r =0 963, P <0 01 respectively). TF expression was a significant risk factor for hepatic metastasis ( P <0 01) and prognosis ( P <0 01). TF expression in HT 29 cells with sense/antisense TFcDNA transfection was more/less than that of the cells without transfection. The invasive ability of HT 29 cells with sense TFcDNA transfection was increased in vitro compared with the untransfected cells, but HT 29 cells with antisense TFcDNA transfection got the contrary change. Conclusions TF may take part in the invasiveandmetastaticprocessofprimarycolorectalcarcinoma,andTFexpressionmaybeanindicatorofhepaticmetastasisandprognosisforcolorectalcarcinomapatients.TFexpressionmayincreasetheinvasiveabilityofHT29cellinvitro.

关 键 词：组织因子 表达 原发性结直肠癌 侵袭 转移 

分 类 号：R735.3[医药卫生—肿瘤]