“彗星”分析法测定人癌体外培养细胞的放射敏感性  

Radiosensitivity detected by "comet" assay in two human tumor cell lines

作　　者：高远红[1] 杨伟志[1] 闫洁[1] 袁智勇[1] 刘新帆[1] 徐国镇[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院放射治疗科

出　　处：《中华肿瘤杂志》2004年第4期217-219,共3页Chinese Journal of Oncology

摘　　要：目的　探讨“彗星”分析法在检测人癌体外培养细胞放射敏感性方面的应用价值。方法应用“彗星”法对人鼻咽高分化鳞状细胞癌CNE 1和肺腺癌 973细胞进行检测 ,以尾力矩作为定量评价指标 ,检测细胞照射后的DNA初始损伤和损伤后的修复能力 ;同时 ,用经典的克隆形成方法测算CNE 1和 973细胞的D0 值和Dq值 ,并对两种实验方法所得结果进行比较。结果　 (1)“彗星”法测定显示 ,CNE 1细胞的初始DNA损伤大于 973细胞 ,差异有显著性 (P <0 .0 1) ,提示CNE 1细胞的放射敏感性高于 973细胞 ;细胞存活曲线检测显示 ,CNE 1细胞的D0 值为 1.6 31,973细胞的D0 值为 1.82 2 ,提示CNE 1细胞的放射敏感性高于 973细胞。两种方法检测结果所反映细胞放射敏感性的趋势一致。(2 )“彗星”分析法检测显示 ,973细胞DNA损伤的半修复时间为 33min ,CNE 1细胞的半修复时间为 4 1min ,提示 973细胞对DNA损伤的修复能力强于CNE 1细胞 ;细胞存活曲线显示 ,973细胞的Dq值为2 .15 2 ,CNE 1细胞的Dq值为 0 .6 2 6 ,提示 973细胞的亚致死损伤修复能力大于CNE 1细胞。两种方法测定结果所反映的损伤修复能力的差异也一致。结论　“彗星”分析法检测CNE 1和 973细胞的DNA初始损伤及修复所反映的放射敏感性 ,与经典细胞存活曲线法所测的放射敏?Objective To evaluate the value of the “comet” assay in detecting the radiosensitivity in human tumor cell lines. Methods The radiation-induced primary DNA damage and repair were detected by the comet assay in CNE-1 and 973 cell lines. The tail moment was used as the end point,to quantitate the primary DNA damage and subsequent repair ability. The cell-survival curve was plotted by the classical colony assay,to detect the D 0 value and Dq value. The results from the above two assays were compared. Results 1. With the increment of irradiation doses,under the same experimental condition,the radiation-induced primary DNA damage was more severe in CNE-1 cells than in 973 cells (P<0.01). From the cell-survival curves,the D 0 value was 1.631 and 1.822 in CNE-1 and CNE-1 973 cells respectively,indicating that CNE-1 cells were more sensitive to irradiation than 973 cells. The radiosensitivity detected by comet assay and by colony assay in the two cell lines tended to be consistent. 2. The half- repair time of 973 and CNE-1 cell line was 33 min and 41 min detected by comet assay,which indicats that the ability of DNA damage and repair in CNE-1 cells was weaker than in 973 cells. The Dq value of the cell survival curve was 2.152 for 973 and 0.626 for CNE-1 cell line detected by the colony assay,which indicates that the sublethal damage repair in 973 cells being much faster than in CNE-1 cells. The repair ability reflected by the results in the two cell lines was consistent. Conclusion The radiosensitivities reflected by the results of the primary DNA damage and repair detected by both comet assay and colony assay in CNE-1 and 973 cells are consistent. It suggests that comet assay is a good method for detecting the radiosensitivity of tumor cells.

关 键 词：“彗星”分析法 放射敏感性 体外培养 DNA损伤 肿瘤细胞 

分 类 号：R73-3[医药卫生—肿瘤]