糖尿病大鼠视网膜组织中Ca^(2+)-Mg^(2+)-ATPase活性变化的实验研究  

Ca^(2+)-Mg^(2+)-ATPase activity in retinal tissue of diabetic rats

作　　者：唐柳萍[1] 杜娟[1] 李向荣[1] 黄艳霞[1] 何剑峰[1] 

机构地区：[1]中国广西自治区柳州市人民医院眼科,545001

出　　处：《国际眼科杂志》2004年第2期250-252,共3页International Eye Science

基　　金：中国广西自治区自然科学基金资助(No.0007036)

摘　　要：目的:观察糖尿病大鼠视网膜组织中钙镁离子三磷酸腺苷酶(Ca2+-Mg2+-ATPase)活性的变化,探讨糖尿病视网膜病变发生发展的有关因素。方法:用孔雀绿比色分析法对糖尿病大鼠发病后不同时期视网膜组织中Ca2+-Mg2+-ATPase活性进行了检测,并与空腹血糖水平、糖基化血红蛋白含量、血脂、视网膜组织中Ca2+和Mg2+含量及糖尿病病程的关系进行了分析。结果:糖尿病大鼠视网膜组织中Ca2+-Mg2+-ATPase活性明显下降,Mg2+含量下降,Ca2+含量增高。Ca2+-Mg2+-ATPase活性与空腹血糖水平、糖基化血红蛋白含量、视网膜组织中Ca2+和Mg2+含量及糖尿病病程密切相关。结论:糖尿病大鼠视网膜组织中Ca2+-Mg2+-ATPase活性下降可能是其糖尿病视网膜病变的病理基础之一。AIM: To study the changes of Ca2+-Mg2+-ATPase activity in retinal tissue of rats with diabetic mellitus, and to analyze relevant factors which effect the occurrence and development of diabetic retinopathy.· METHODS: Ca2+-Mg2+-ATPase activity was detected with peacock-green calorimetric analysis in retinal tissue of diabetic rats at various time of diabetic course, and the relation of Ca2+-Mg2+-ATPase activity with level of fasting blood glucose (FBG), hemoglobin glycosylation (HbA1c) content, concentration of Ca2+ and Mg2+ in the retinal tissue, and the course of disease was studied.· RESULTS: Ca2+-Mg2+-ATPase activity was significantly decreased, Mg2+ content decreased and Ca2+ increased in retinal tissue of diabetic rats. There was a marked relation between Ca2+-Mg2+-ATPase activity and the factors including FBG, HbA1c, blood lipid , content of Ca2+ and Mg2+ in retinal tissue, and course of disease.· CONCLSUION: The decrease of Ca2+-Mg2+-ATPase activity in retinal tissue of diabetic rats may be one of pathologic basis in occurrence of diabetic retinopathy.

关 键 词：糖尿病 大鼠 视网膜 钙离子 镁离子 三磷酸腺苷酶 血糖 

分 类 号：R774.1[医药卫生—眼科]