三七总皂甙和银杏叶提取物预防急性氧中毒的实验研究  

EXPERIMENTAL STUDIES OF PANAX NOTOGINSENG SAPONINES AND GINKGO BILOBA EXTRACTS ON PREVENTING ACUTE OXYGEN TOXICITY

作　　者：李润平[1] 曹永兵[2] 张汉明[3] 陶恒沂[1] 孙学军[1] 卢玲[1] 许熊飞[1] 

机构地区：[1]第二军医大学海军医学系航海医学教研室,上海200433 [2]第二军医大学药学院药理学教研室,上海200433 [3]第二军医大学药学院生药学教研室,上海200433

出　　处：《中国应用生理学杂志》2004年第2期201-204,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助课题 ( 3 990 0 172 ) ;军队"九五"科研课题资助 ( 96M 0 75 );第二军医大学联合攻关课题资助

摘　　要：目的 :研究三七总皂甙和银杏叶提取物对急性氧中毒的预防作用及其可能机制。方法 :分别给小鼠连续腹腔注射三七总皂甙和银杏叶提取物 5d后 ,在 5 0 0kPa高压氧中暴露 6 0min ,观察惊厥潜伏期、惊厥次数、惊厥间隔时间等指标 ;另外测定高压氧暴露 15min后脑组织中活性氧单位、脂质过氧化物、一氧化氮、谷胱甘肽的含量和过氧化氢酶、谷胱甘肽过氧化物酶、单胺氧化酶的活性。结果 :三七总皂甙和银杏叶提取物可以明显延长氧惊厥潜伏期和惊厥间隔时间 ,减少惊厥次数 ;降低高压氧暴露后脑组织中脂质过氧化物、一氧化氮的含量 ,使活性氧单位、谷胱甘肽含量和谷胱甘肽过氧化物酶活性保持在较高的水平 ;对过氧化氢酶和单胺氧化酶活性的影响则不显著。结论 :三七总皂甙和银杏叶提取物可以有效预防急性氧中毒 ,其机制可能与它们的抗氧化活性有关。Aim: To investigate the preventive effects of Panax notoginseng saponines (PNS) and Ginkgo biloba extracts (GbE) on acute oxygen toxicity and the possible mechanisms. Methods: Mice were injected intraperitoneally with PNS and GbE for 5 days, then were exposed to 500 kPa hyperbaric oxygen (HBO) for 60 min, the convulsion latency, times and interval were observed. Moreover, reactive oxygen (RO) unit, MDA, NO, GSH levels and GSH-Px, CAT, MAO activities of mice brain were determined after they were exposed to HBO for 15 min. Results: PNS and GbE could markedly prolong the convulsion latency and interval, reduce convulsion times, decrease contents of MDA and NO in mice brain, keep RO unit, GSH and GSH-Px at higher levels, but had no effects on CAT and MAO activities. Conclusion: PNS and GbE could effectively prevent acute oxygen toxicity, which were related to their antioxidant activities.

关 键 词：三七总皂甙 银杏叶提取物 氧中毒 中毒机制 抗氧化活性 

分 类 号：R845.23[医药卫生—航空、航天与航海医学] R285[医药卫生—临床医学]