皮肤鳞状细胞癌血管生成过程中管腔超微形态学研究  

Ultrastructural Changes of Lumen Formation During the Angiogenesis in Squamous Cell Carcinoma

作　　者：劳力民[1] 单慧[2] 朱可建[1] 茅晓红[1] 孙国钧[1] 

机构地区：[1]浙江大学医学院附属第二医院皮肤科,浙江杭州310009 [2]浙江大学医学院附属口腔医院,浙江杭州310006

出　　处：《中国皮肤性病学杂志》2004年第5期273-275,共3页The Chinese Journal of Dermatovenereology

基　　金：浙江省中医药管理局科研基金项目 (2 0 0 2C0 56)

摘　　要：目的　研究皮肤鳞状细胞癌血管生成过程中微血管内皮细胞的变化及肿瘤细胞血管内皮生长因子抗体(VEGF)的表达状况。方法　采用透射电镜和免疫组化法。结果　内皮细胞内囊泡形成 ,继而与胞膜融合 ,形成胞内管腔 ;毛细血管芽内部的内皮细胞凋亡 ,扩大为细胞间管腔。肿瘤细胞VEGF强阳性着色 ,新形成的血管内皮细胞Ⅷ因子抗体阳性。结论　内皮细胞的凋亡可能是鳞状细胞癌新生血管管腔形成的重要步骤 ,血管生成受血管内皮生长因子调控。Objective To investigative the morphological changes of capillary endothelial cells(ECs) during the angiogenesis in squamous cell carcinoma(SCC),and the expression of vascular endothelial cell growth factor (VEGF) in tumor cells.Methods Transmission electron microscope and immunohistochemistry were used.Results The intracellular lumen formation occurred through the vacuoles coalescing within the cytoplasm and subsequent directional fusion with the plasma membrane;Apoptosis of ECs within the capillary sprout resulted in expansion of intercellular space into a lumen.The positive staining of VEGF in tumor cells and Ⅷ factor in new ECs was seen. Conclusion The results indicated that apoptosis of ECs might play an important role in lumen formation during the angiogenesis in SCC.VEGF might involve in the regulation of angiogenesis.

关 键 词：皮肤鳞状细胞癌 血管生成 超微结构 形态学 血管内皮生长因子 管腔 

分 类 号：R739.5[医药卫生—肿瘤]