Isolation and Characterization of the Wheat cDNA Fragments Involved in Salt Stress  

作　　者：MAWen-shi Gui-ping SHENYin-zhu HUANGZhan-jing 

机构地区：[1]CollegeofLifeScience,HebeiNormalUniversity,Shijiazhuang050016,P.R.China [2]BiologyandScienceTechnologyDepartment,TangshanTeacher＇sCollege,Tangshan063000,P.R.China

出　　处：《Agricultural Sciences in China》2004年第3期173-177,共5页中国农业科学（英文版）

基　　金：supported by the National Natural Science Foudation of China(30070471);the Natural Science Foundation of Hebei Province(301103).

摘　　要：cDNA-AFLP(amplified fragment length polymorphism) is used to isolate genes differentiallyexpressed in salt-stressed and unstressed wheat lines RH8706-49 and H8706-34 derivedfrom a single seed, representing a salt-stress resistant (SR) and salt-stress sensitive(SS) line, respectively. About 88.1% cDNA fragment are expressed in all the four samples,11.9% are different between the samples. 68 cDNA fragments were cloned, of which 35 weresubject to sequence analysis. Database searches indicate that 11 cDNA fragment show highhomology to known genes, which mainly include proteins related to ion transport, signaltransduction and oxidative stress. The remaining 24 cDNA show no detectable homology toknown genes, suggesting that they probably represent novel genes.cDNA-AFLP(amplified fragment length polymorphism) is used to isolate genes differentiallyexpressed in salt-stressed and unstressed wheat lines RH8706-49 and H8706-34 derivedfrom a single seed, representing a salt-stress resistant (SR) and salt-stress sensitive(SS) line, respectively. About 88.1% cDNA fragment are expressed in all the four samples,11.9% are different between the samples. 68 cDNA fragments were cloned, of which 35 weresubject to sequence analysis. Database searches indicate that 11 cDNA fragment show highhomology to known genes, which mainly include proteins related to ion transport, signaltransduction and oxidative stress. The remaining 24 cDNA show no detectable homology toknown genes, suggesting that they probably represent novel genes.

关 键 词：Common wheat CDNA-AFLP Salt stress MUTANT 

分 类 号：S512.103[农业科学—作物学]