侵染马铃薯的一个Tospovirus混合分离物的鉴定、纯化及多抗血清制备  

Identification, purification and antiserum preparation of Tospovirus isolate affecting potato

作　　者：丁铭[1] 张丽珍[1] 方琦[1] 李婷婷[1] 苏晓霞[1] 李展[1] 张仲凯[1] 

机构地区：[1]云南省农业生物技术重点实验室,云南昆明650223

出　　处：《西南农业学报》2004年第B05期160-162,共3页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(30160005);国家科技部农业科技成果转化资金项目

摘　　要：在昆明地区栽培的马铃薯病株上分离鉴定出含有番茄斑萎病毒属(Tospovirus)番茄斑萎病毒(Tomatospotted wilt virus,ISWV)和花生环斑病毒(Groundnut ringspot virus,GRSV)。利用蔗糖垫及差速离心法提纯了该混合分离物,提纯病毒免疫家兔5次,得到TSWV和GRSV的混合抗血清,所制备的抗血清经间接ELISA测定效价为1:2560。Tospovirus, Tomato spotted wilt virus and Groundnut ringspot virus, isolates were obtained and identified from potato plants in Kunming District. The isolates were isolated and purified from plants by differential speed and 20% (w/v) sucrose. The virions was purified. The purified virions immunized rabbit 5 times. The titre of antiserum was 1:2560 in indirect ELBA test.

关 键 词：马铃薯 病株 分离 鉴定 番茄斑萎病毒 花生环斑病毒 TSWV GRSV 抗血清 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]