头孢哌酮钠的直接和间接示波测定  

Direct and Indirect Oscillographic Determination of Cefoperazone Sodium

作　　者：倪宏刚[1] 郑建斌[1] 

机构地区：[1]西北大学分析科学研究所,陕西西安710069

出　　处：《分析测试学报》2004年第3期80-82,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20275030) ;教育部科学技术研究重点项目(0287) ;教育部高等学校骨干教师资助计划(GG-150-71006-2877) ;陕西省教育厅专项基金项目(01JK076)

摘　　要：在pH4.2HAc -NaAc缓冲溶液中 ,头孢哌酮钠在示波图阴极支 -1.48V(vsSCE)处产生一个灵敏切口 ;而在0.2mol/LNaOH底液中 ,头孢哌酮钠与 β_环糊精(β_CD)形成包结物使 β_CD在示波图上产生的切口变浅。根据这些示波特性 ,建立了测定头孢哌酮钠注射剂中头孢哌酮钠含量的直接和间接二次微分简易示波伏安法。实验结果表明 :直接测定头孢哌酮钠的线性范围为1.2×10 -5～2.2×10 -4mol/L ,检出限为6.0×10 -6mol/L ,对3.000×10 -5mol/L进行5次测定的RSD为2.1 % ;而间接测定的线性范围为2.0×10 -6～9.0×10 -5mol/L ,检出限8.0×10 -7mol/L ,对2.500×10 -6mol/L进行5次测定的RSD为2.7 %。与直接法相比较 。In HAc-NaAc buffer solution(pH4.2),a sensitive incision at-1.48V(vs.SCE)of caˉthodic branch of dV/dt-Vcurve could be observed in the presence of cefoperazone sodium,while a shalˉlow incision could be observed in the presence of5.0×10 -5 mol/Lβ-CD+0.2mol/L NaOH solution with the increase of cefoperazone sodium concentration due to the inclusion formed between cefoperazone sodiumandβ-CD.Based on these oscillographic characteristics,a direct and an indirect second order difˉferential simple oscillographic voltammetry for the determination of cefoperazone sodium in injection were developed in pH4.2HAc-NaAc solution and in5.0×10 -5 mol/Lβ-CD+0.2mol/L NaOH solution,respectively.Experimental results showed that the linear range and the detection limit of the direct method were1.2×10 -5 ～2.2×10 -4 mol/L and6.0×10 -6 mol/L,respectively,and the RSD was2.1%for3.000×10 -5 mol/Lcefoperazone sodium(n=5).The linear range and the detection limit of the indirect method were2.0×10 -6 ～9.0×10 -5 mol/L and8.0×10 -7 mol/L,respectively,and the RSD was2.7%for2.500×10 -6 mol/Lcefoperazone sodium(n=5).Compared with the direct method,the indiˉrect one has broader linear range and lower detection limit.

关 键 词：示波分析 示波测定 二次微分简易示波伏安法 Β-环糊精 头孢哌酮钠 

分 类 号：O657.14[理学—分析化学] R978.1[理学—化学]