奥曲肽对胰腺癌细胞转染SST 2后的作用  

Effect of octreotide on pancreatic cancer cells transfected with SST 2 gene

作　　者：刘正人[1] 吴高松[1] 杜志勇[1] 王大禹[1] 秦仁义[1] 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,湖北武汉430030

出　　处：《中国普通外科杂志》2004年第6期424-427,共4页China Journal of General Surgery

基　　金：国家自然科学基金 (30 2 71 4 73) ;湖北省自然科学基金(2 0 0 0J0 68)

摘　　要：目的　将生长抑素二型受体 (somatostatinreceptortype 2 ,SST 2 )基因转染至胰腺癌细胞株PC 3细胞 ,探索奥曲肽对阳性表达SST 2的PC 3细胞凋亡影响。方法　采用脂质体 (Liposome)将SST 2基因转染至PC 3细胞 ,免疫组织化学染色检测其转染效果。MTT比色法及流式细胞术检测不同浓度梯度奥曲肽对PC 3细胞转染SST 2基因前后凋亡的影响。结果　胰腺癌细胞转染SST 2后 ,不同浓度奥曲肽 (0 .2 ,0 .4,0 .8μg/ml)杀伤已转染SST 2的PC 3细胞的能力较杀伤未转染SST 2的PC 3细胞的能力均有明显增强 (均P <0 .0 5 )。结论　生长抑素用于治疗PC 3细胞效果不佳可能与SST 2下调有关 ,转染SST 2后可增加奥曲肽对PC 3细胞的杀伤能力 ,这为临床利用SST 2基因转染加生长抑素类似物治疗胰腺癌提供了实验基础。Objective To observe the effect of octreotide on human pancreatic cancer cells (PC-3) apoptosis after PC-3 transfected with somatostatin receptor type 2 (SST 2) gene. Methods SST 2 was transfected into PC-3 by liposome,the result of transfection was detected by immunohistochemistry. The apoptosis of PC-3 induced by using different dosage of octreotide were detected by MTT assay and flow cytometry. Results The effects of killing PC-3 by different dosage (0.2,0.4 and 0.8μg/ml ) of octreotide in transfected groups were significantly stronger than those in non-transfected groups(P<0.05). Conclusions Down-regulation of SST 2 is probably the major cause of octreotide has little effect on pancreatic cancer cells. Transfecting SST 2 can strengthen the ability of octreotide on killing PC-3. It may provides an experimental base for using both of octreotide and transfection SST 2 on treating pancreatic cancer clinically.

关 键 词：胰腺肿瘤/药物疗法 奥曲肽/治疗应用 细胞转染 受体 生长抑素 

分 类 号：R735.9[医药卫生—肿瘤]