毛细管电泳高频电导法快速分离检测混合氨基酸  

A Rapid Separation and Determination of Amino Acids by Capillary Electrophoresis with a High Frequency Conductivity Detector

作　　者：翟海云[1] 蔡沛祥[1] 陈缵光[2] 李冠宏[2] 叶欣[1] 莫金垣[1] 

机构地区：[1]中山大学化学与化学工程学院,广州510275 [2]中山大学中山医学院,广州510089

出　　处：《高等学校化学学报》2004年第6期1037-1039,共3页Chemical Journal of Chinese Universities

基　　金：广东省自然科学基金重点项目 (批准号 :0 2 180 8)资助

摘　　要：A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H3BO3/0.10 mmol/L CTAB/0.25 mmol/L β-CD（pH=11.0） at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L（S/N=3）. [WT5HZ]A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide(CTAB) was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H_3BO_3/0.10 mmol/L CTAB/0.25 mmol/L β-CD(pH=11.0) at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L(S/N=3). [WT5HZ]

关 键 词：反向毛细管电泳 高频电导检测 非接触式电导检测 氨基酸 

分 类 号：O657[理学—分析化学]