兔慢性高眼压模型的建立  

Establishment of ocular hypertension model in rabbits

作　　者：王昌鹏[1] 杨新光[1] 王晓娟[2] 李养军[1] 

机构地区：[1]第四军医大学唐都医院眼科,陕西西安710038 [2]第四军医大学口腔医学院药剂科,陕西西安710033

出　　处：《第四军医大学学报》2004年第7期606-609,共4页Journal of the Fourth Military Medical University

基　　金：全军医药卫生科研基金 (0 1MA1 97)

摘　　要：目的 :建立一种持续时间较久的慢性高眼压动物模型 .方法 :新西兰白兔 1 0只左眼为对照眼 ,右眼为模型眼 .模型眼前房注入 3g/L复方卡波姆溶液制作成慢性高眼压模型 ,对照眼只作前房穿刺 .每日测量眼压 ;在高眼压模型前和高眼压持续 1 ,2及 4wk共 4个时间点 ,检测ERG的b波、Ops波的振幅 ;高眼压持续 4wk后检测视网膜节细胞 (retinalgan glioncells,RGCs)数目的变化 .结果 :①模型眼的眼压(4 .4 7± 1 .4 1 )kPa ,明显高于对照眼 (2 .0 8± 0 .2 5 )kPa(t =4 .798,P =0 .0 0 1 ) .②高眼压持续 1 ,2及 4wk后 ,对照眼b波的振幅为 (2 1 1 .4 9± 5 7.2 2 ,2 0 7.86± 5 1 .89,2 0 1 .77± 5 6 .4 7)μV ,Ops波的振幅为 (77.4 5± 2 4 .0 8,73.6 1± 8.87,79.6 6± 2 1 .94 ) μV ;模型眼b波的振幅为 (1 4 9.0 8± 2 0 .5 5 ,1 2 1 .1 2± 2 7.99,81 .82± 77.6 6 ) μV ,Ops波的振幅为(5 0 .0 9± 9.77,39.99± 8.98,33.1 2± 7.6 7) μV ;二者间b波、Ops波的振幅有非常显著性差异 (P值均为 0 .0 0 0 ) ,模型眼b波和Ops波的振幅在高眼压前和高眼压持续 1 ,2及 4wk时有非常显著性差异 (P值均为0 .0 0 0 ) ,并且随高眼压持续时间的延长有进行性降低的趋势(P值分别为 0 .0 0 1 ,0 .0 0 0 ) .AIM: To establish a stable ocular hypertension (OHT) model. METHODS: Left eyes of 10 rabbits were used as control eyes, their right eyes as model eyes. The OHT was induced by injecting 3 g/L carbomer into anterior chamber of the model eyes and paracentesis was performed in the control eyes but without the injection of carbomer. The intraocular pressure (IOP) was measured every day and the amplitudes of b wave and Ops wave were measured before injection and one, two and four weeks after. Four weeks after the injection, retinal ganglion cells (RGCs) were counted. RESULTS: ①The IOP of model eyes was (4.47±1.41) kPa, significant higher than that (2.08±0.25) kPa of control eyes ( t =4.798, P =0.001). ②The amplitudes of b wave in the control eyes and in model eyes were (211.49±57.22, 207.86±51.89, 201.77± 56.47) μV, and (149.08±20.55, 121.12±27.99, 81.82±77.66)μV, respectively. The amplitudes of Ops wave in control eyes and in model eyes were (77.45±24.08, 73.61± 8.87, 79.66±21.94)μV. and (50.09±9.77, 39.99± 8.98, 33.12±7.67)μV, respectively. The amplitudes of b wave and Ops wave in control were significantly different from those in model eyes ( P =0.000). The amplitudes of b wave and Ops wave in model eyes were also significantly different at different time points ( P =0.000). The amplitudes of b wave and Ops wave in model eyes tended to decline during OHT ( t =0.001, P =0.000). ③ RGCs of control eyes was (302.30±23.99)/10 mm, more than those of model eyes (89.20±10.86)/10 mm ( t = 23.685 , P =0.000). CONCLUSION: The detection of IOP, function and configuration of retina proves that the OHT induced by injection of 3 g/L carbomer into anterior chamber of rabbit is a stable OHT model.

关 键 词：琅高压 视网膜电图 视网膜节细胞 卡波姆 

分 类 号：R775.2[医药卫生—眼科]