一个肝癌相关基因的生物信息学及其基因表达分析  

Bioinformatics and expression profile analysis of a liver cancer associated gene

作　　者：於莉[1] 韩泽广[1] 黄健 

机构地区：[1]华东理工大学生物工程学院,上海200237 [2]国家人类基因组南方研究中心,上海201203

出　　处：《肿瘤防治杂志》2004年第3期225-228,共4页China Journal of Cancer Prevention and Treatment

基　　金：国家 973计划资助项目子课题 (G19980 5 10 0 5 );国家自然科学基金资助项目 ( 3 0 0 70 85 2 )

摘　　要：目的 :筛选并克隆肝癌相关基因 ,探讨肝癌发病机制。方法 :在国家人类基因组南方研究中心肝癌基因表达谱及其功能研究的工作基础上 ,发现 1个未知功能基因 (暂命名LLC基因 ) ,其DNA拷贝数在肝癌基因组中呈降低的趋势。本研究利用生物信息学和RT PCR的方法 ,对其进行功能预测并在转录组水平检测该基因的表达。结果 :LLC基因定位于染色体8p2 3 3区段 ,全长为 670 6bp ,含有 2个外显子 ,1个内含子 ,其开放阅读框长为 1871bp ,编码 1个含有 62 3个氨基酸的蛋白。该基因在 67%( 16/2 4)肝癌组织中呈现下调趋势。同时其在人体内多种组织均有表达。结论OBJECTIVE:To screen and clone novel genes related to liver cancer.METHODS:Based on the former research of liver cancer, an unknown function gene related to liver tumor was identified and named as LLC gene,which copy numbers was lower in hepatocellular carcinoma (HCC) than in normal tissue in genome level.The LLC mRNA expression profile in 24 pair samples of HCC and non-HCC was confirmed by bioinfomatics and Reverse Transcriptase-PCR.RESULTS:LLC gene localized on chromosome 8p23.3 and the span was 6 706 bp with 2 exons and 1 intron.The open reading frame was 1 871 bp,and encoded a deduced protein containing 623 amino acid residues.LLC gene was low-expressed in 67% (16/24) HCC.Meanwhile,it expressed in many normal tissues.CONCLUSION:LLC gene may be a novel tumor suppressor gene in liver cancer.

关 键 词：肝肿瘤/遗传学 基因表达 基因组 人 医学信息学 逆转录聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]