燃煤型砷中毒皮损中2个DNA微卫星位点的变化  

Detection of Microsatellite Instability in Lesions from Patients with Coal Arsenic Poisoning

作　　者：袁伟[1] 张信江[1] 瓦庆彪[1] 郑庭铭[1] 肖和[1] 杜辉 周运书 

机构地区：[1]遵义医学院附属医院皮肤性病科,贵州563003 [2]贵州省黔西南州疾病控制中心

出　　处：《中华皮肤科杂志》2004年第2期88-90,共3页Chinese Journal of Dermatology

基　　金：中华医学会皮肤科分会基金(200130);贵州省省长资金(C-197)

摘　　要：目的探讨微卫星不稳定性及杂合性丢失在燃煤型砷中毒患者皮肤病变发展中的作用。方法依据皮肤病理组织学检查结果对燃煤型砷中毒患者进行分组,选取TP53.PCR15、D9S3192个微卫星多态性标记,采用PCR扩增-变性聚丙烯酰胺凝胶电泳-银染法检测其微卫星不稳定性情况。结果34份燃煤型砷中毒患者皮损组织微卫星不稳定性的发生率为32.4%(11/34),杂合性丢失的发生率为14.7%(5/34),微卫星不稳定性与临床分度、病理分型之间统计学分析差异无显著性(P>0.05)。结论微卫星不稳定性和杂合性丢失可能在砷中毒皮损癌变的发生发展中起一定的作用。Objective In order to elucidate the significance of both microsatellite instability(MSI) and loss of heterozygosity (LOH) in the pathogenesis of arsenic-induced skin lesions, especially skin carcinoma, the presence of MIS and LOH at two loci [chromosome 9p21 (D9S319, p16) and 17p13.1 (TP53.PCR15, p53)] was evaluated. Methods Using polymerase chain reaction(PCR)-denatured polyacrylamide gel electrophoresis-silver staining, MSI and LOH were detected in a total of 34 samples of arsenic-induced skin lesions, and the results were compared with the clinicopathological parameters. Results MSI was found in 32.4% (11/34), and LOH was found in 14.7%(5/34) of 34 samples of arsenic-induced skin lesions. There were no significant differences between the presence of MSI and clinical severity or pathological grades (P > 0.05). Conclusions MSI and LOH may play a certain role in the carcinogenesis and progression of arsenic-induced skin lesions.

关 键 词：燃煤型砷中毒 微卫星不稳定性 杂合性丢失 皮肤病 微卫星多态性 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]