凋亡相关蛋白在胃癌细胞凋亡中的作用研究  

Effects of apoptosis-related protein on apoptosis of gastric cancer cells

作　　者：杨柳芹[1] 房殿春[1] 罗元辉[1] 

机构地区：[1]第三军医大学西南医院全军消化病专科中心,重庆400038

出　　处：《中华消化杂志》2004年第1期4-6,共3页Chinese Journal of Digestion

基　　金：全军"十五"科研基金资助 ( 0 1MA1 72 )

摘　　要：目的　研究核转录因子 (NF) κB、生存素 (survivin)、Bcl 2和Caspase3在肿瘤坏死因子相关凋亡诱导配体 (TRAIL)诱导的胃癌细胞凋亡中的作用。方法　应用细胞培养和流式细胞仪检测的方法 ,观察胃癌细胞SGC 790 1、MKN2 8、AGS、MKN45经TRAIL作用后的细胞凋亡率 ,Westernblot方法分析 4种胃癌细胞中NF κB、生存素、Bcl 2和Caspase3的表达。结果　TRAIL 50ng/ml作用于胃癌细胞 2 4h后 ,MKN2 8、MKN 45、AGS和SGC 790 1细胞的凋亡率分别为 2 4.0 5% ,7.83 % ,8.0 5%和3 .17%。TRAIL 3 0 0ng/ml作用于胃癌细胞 2 4h后 ,MKN2 8、MKN 45、AGS和SGC 790 1细胞的凋亡率分别为 3 6.0 5% ,2 0 .2 7% ,16.50 %和 11.80 %。Westernblot结果显示 ,SGC 790 1细胞NF κB、生存素的表达高于MKN2 8细胞 (P <0 .0 5) ,与Bcl 2的表达无明显差异。MKN2 8细胞Caspase3的表达高于SGC 790 1细胞 (P <0 .0 5)。结论　TRAIL具有选择性诱导肿瘤细胞凋亡的特性 ,肿瘤细胞对TRAIL的耐药现象可能与凋亡抑制因子生存素和NF κB的表达增强以及Caspase3Objective To investigate the effects of nu cleus transcription factor (NF)-κB, survivin, Bcl-2 and Caspase-3 on apoptos is of gastric cancer cells induced by tumor necrosis factor related apoptosis in ducing ligand(TRAIL). Methods Gastric cancer cells were cultured in RPMI-1640 and the proportions of apoptosis were analyzed by flow cytometry. The expressions of N F-κB, survivin, Bcl-2 and Caspase-3 in gastric cancer cells were evaluated b y Western blot. Results After gastric cancer cells were exposed to 50 ng/ml TRAIL f or 24 hours, the proportions of apoptosis were 24.05% in line MKN28, 7.83% in MK N45, 8.05% in AGS and 3.17% in SGC-7901 respectively; after 300 ng/ml TRAIL for 24 ho urs, the proportions of apoptosis were 36.05% in MKN28, 20.27% in MKN45, 16.50% in AGS and 11.80% in SGC-7901 respectively. The expressions of NF-κB and surv ivin under Western blot were lower in MKN28 than that in MKN45, AGS and SGC-79 0 1. In contrast, the expression of Caspase-3 was higher in MKN28 than that in MK N45, AGS a nd SGC-7901. The expression of Bcl-2 had no difference among the 4 lines of ga stric cancer cells. Conclusions The anti-tumor sensitivity of TRAIL to gastric cancer c ell may be associated with both increased expressions of NF-κB and survivin an d decreased expression of Caspase-3.

关 键 词：凋亡相关蛋白 胃癌 细胞凋亡 细胞培养 流式细胞仪 细胞因子 

分 类 号：R735.2[医药卫生—肿瘤]