电磁脉冲辐射对原癌基因c-fos调控区的影响  

Effects of EMP on Regulatory Regions of c-fos Gene

作　　者：赵梅兰[1] 曹晓哲[1] 王德文[1] 刘杰[1] 哈小琴[2] 吴丹莉[2] 张燕[1] 

机构地区：[1]军事医学科学院放射医学研究所实验病理研究室,北京100850 [2]军事医学科学院放射医学研究所实验血液室,北京100850

出　　处：《中华物理医学与康复杂志》2004年第2期65-68,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：解放军"十五"指令课题基金资助 (No .0 1L0 2 3)

摘　　要：目的　通过研究电磁脉冲 (electromagneticpulse ,EMP)辐射对原癌基因c fos调控区域的影响 ,探讨其诱导细胞功能改变的机制。方法　构建c fos启动区氯霉素乙酰化转移酶 (chloramphenicolacetyl transferase,CAT) ,然后转染HeLa癌细胞株 ,高场强EMP模拟源 (有界波模拟源 )辐射细胞 ,EMP场强为6× 10 4V/m ,脉冲上升时间为 2 0ns ,脉宽为 3 0 μs ,频率为 2 .5个脉冲 /min ,辐射 2min ,处理后 2 0min时观察细胞中CAT的活性。结果　EMP辐射转染的细胞后 2 0min ,转染了P50 0 ( -711～ -2 2 3bp)和P2 50 ( -3 62～-10 0 )的Hela细胞其CAT活性明显高于对照水平的活性 (P <0 .0 1)。结论　EMP辐射可引起c fos基因调控区的 -3 62～ -2 2 5明显改变。Objective To determine whether regulatory regions of c-fos gene were responsive to electromagnetic pulse (EMP) and to explore the possible underlying mechanisms. MethodsHela cells were transiently transfected with plasmids containing upstream regulating regions of c-fos up to -700bp, coupled with the prokaryotic reporter gene CAT(chloramphenical acetyl-transferase). Cells were exposed to EMP(simulator which provides 5 pulses/2 min with a high electric field intensity 60kV/m, 20-nsec rise time and 30ms pulse width.). CAT was measured by enzymatic determinations. Acetylated 14 C-chlormphenicol was measured by thin layer chromatography. ResultsCAT expression above control levels in transfected cells (region -362～-225bp) was observed at 20 min following exposure to EMP,especially in P500(-711～-223bp)and P250(-362～-100)group( P <0.01). ConclusionEMP affects the regulation regions of c-fos gene.

关 键 词：电磁脉冲 原癌基因 c-fos基因调控区 氯霉素乙酰化转移酶 HELA细胞 

分 类 号：R363[医药卫生—病理学]