塞来昔布对二甲基肼诱导的大鼠畸变隐窝灶的预防作用  

Chemopreventive effects of celecoxib on rat colon aberrant crypt foci induced by dimethylhydrazine

作　　者：李军[1] 吕愈敏[1] 金珠[1] 董秀云[1] 

机构地区：[1]北京大学第三医院消化科,100083

出　　处：《中华消化杂志》2004年第3期151-153,共3页Chinese Journal of Digestion

摘　　要：目的　明确塞来昔布 (选择性COX 2抑制剂 )对二甲基肼 (DMH)诱导的大鼠畸变隐窝灶(ACF)的化学预防作用 ,并与舒林酸 (一种非甾体类抗炎药 )进行比较。方法　 32只 8周龄雌性SD大鼠 ,随机分为 4组 ,每组 8只。第 1组单次腹腔注射DMH(每千克体重 12 0mg) ;第 2组单次腹腔注射生理盐水 (1ml/只 ) ;第 3组先舒林酸 (每千克饲料 32 0mg)喂养 7d ,然后注射DMH ;第 4组先塞来昔布(每千克饲料 15 0 0mg)喂养 7d ,然后注射DMH。注射DMH 5周后处死大鼠 ,美蓝染色后计数每只大鼠大肠中ACF和畸变隐窝 (AC)个数。结果　第 1组平均每只大鼠结肠中ACF和AC分别为 (182 .4 0± 93.4 3)和 (2 6 2 .80± 197.80 )个。第 2组没有发现ACF。第 3组ACF和AC分别为 (91.2 5± 4 8.98)和(139.6 0± 6 8.5 2 )个 ,与第 1组相比差异有显著性 (P <0 .0 5 )。第 4组ACF和AC分别为 (6 5 .83± 38.5 4 )和 (10 6 .0 0± 6 1.0 3)个 ,与第 1组相比差异有显著性 (P <0 .0 1)。第 3组与第 4组相比 ,ACF及AC个数差异无显著性。结论　选择性COX 2抑制剂塞来昔布对实验诱导的大鼠ACF具有预防作用 ,其作用与舒林酸相当。Objective An investigation was conducted to assess the chemopreventive effects of celecoxib, a selective cyclooxygenase(COX)-2 inhibitor, on a rat colon carcinogenesis model induced by dimethylhydrazine (DMH). The results were compared with those of sulindac. Methods Thirty-two 8-week-old female Sprague-Dawley rats were randomly divided into four groups (n=8 each):Group A rats were treated with DMH(120 mg/kg wt, single subcutaneous injection) alone and group B rats were treated with saline alone. Group C rats were pre-treated with sulindac (320 mg/kg feedstuff) and group D rats pre-treated with celecoxib (1500 mg/kg feedstuff) for 7 days before DMH initiation. The animals were killed at the end of the experiment (week 5) and the aberrant crypt foci(ACF) and aberrant crypt(AC) of the colonic mucosa were assessed after being stained with methylene blue. Results In group A(DMH only), the average numbers of ACF and AC were 182.4±93.43 and 262.8±197.8 respectively. In group B (saline group) rats, no ACF was found. In group C (sulindac group) rats, the average numbers of ACF and AC were 91.25±48.98 and 139.60±68.52 respectively, both of them were decreased significantly as compared with those in Group A (P<0.05). In group D(celecoxib group) rats, the average numbers of ACF and AC were 65.83±38.54 and 106.0±61.03 respectively, significantly lower than those in Group A (P<0.01). No significant difference was found between group C and D. Conclusions Like sulindac, celecoxib may have chemopreventive effects against rat colon carcinogenesis induced by DMH.

关 键 词：塞来昔布 二甲基肼 大鼠 畸变隐窝灶 大肠癌 化学预防 癌前病变 

分 类 号：R735.34[医药卫生—肿瘤]