毛细管参考链介导的构象分析法在HLA-DRB1等位基因分型中的应用  

HLA-DRB1 allele assignments by reference strand mediated conformation analysis using capillary-based genetic analyzer

作　　者：毕颎[1] 陈规划[2] 刘泽寰[3] 徐安龙[3] 黄丽英[4] 

机构地区：[1]中山大学附属第一医院,广州510080 [2]中山大学附属第三医院,广州510630 [3]中山大学生命科学院,广州510275 [4]中山大学达安基因诊断中心,广州510080

出　　处：《中国免疫学杂志》2004年第5期326-327,340,共3页Chinese Journal of Immunology

摘　　要：目的 :探讨应用参考链构象分析法对HLA DRB1等位基因分型的准确性及实用性。方法 :应用毛细管参考链介导的构象分析法建立 31个等位基因的标准迁移率 ,对 5 0例样本进行分型 ,并与SBT法检测结果进行比较。结果 :5 0例样本中 4 7 5 0与测序法测得的等位基因一致 ,以DRB1 0 80 32、DRB1 0 90 12为参考链无法检测出DRB1 110 1、DRB1 14 0 3。结论 :毛细管参考链介导的构象分析法具有准确性高、分辨率高、成本低、高通量的特点 。Objective:To investigate the accuracy and application of reference strand mediated conformation analysis(RSCA) using capillary-based genetic analyzer in HLA-DRB1 allele assignments.Methods:The standard mobility of 31 alleles were established through reference strand mediated conformation analysis using capillary-based genetic analyzer. 50 samples were typing by RSCA in contrast with SBT.Results:Of the 47/50 samples typed , there was complete concordance between HLA-DRB1 capillary RSCA and SBT. It was observed that the DRB1 *08032 and DRB1 *09012 fluorescently labelled reference did not allow discrimination of the following alleles DRB1 *1101、DRB1 *1403.Conclusion:Capillary RSCA is used in HLA typing with accuracy, high resolution, high throughput and low cost. It is desirable to be applied after further improvement.

关 键 词：参考链介导的构象 等位基因 HLA—DRB1分型 

分 类 号：R446.62[医药卫生—诊断学] Q503[医药卫生—临床医学]